This chapter provides an overview of the common types of defects found in various structural materials and joints in aircraft. Materials manufacturing methods (including large-scale production) have been established in the aircraft industry. However, as will be seen in this chapter, manufacturing defec...
The most prevalent plastic valve material, Grade 1 Type 1 PVC (polyvinylchloride), has been used successfully for over 35 years in such areas as chemical processing, wastewater treatment, industrial plating and electronics manufacturing. It carries a pressure rating of approximately 150 psi -- at ...
Cells plated on six-well plates were trans- fected 12–24 h after plating when they had reached 50– 80% confluency, and then analyzed 24–48 h following transfection. To depolymerize microtubules, cells were treated for 6 h at 37 °C with 0.5 μM nocodazole (Sigma, from a 3.3 mM ...
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Cells were resuspended in 100 µl LB-DAP (0.3 mM DAP) and mixed before spot plating onto LB-DAP (0.3 mM DAP) agar. After incubation for 5–7 h at 37 °C, cells were resuspended in 2 mL of LB. Cells were washed twice with LB to remove the residual DAP resulting in the ...
Cells were differentiated the day after plating by 10 μM All-trans retionoic acid (RA; Sigma Aldrich). After 5 days, the cells were washed three times with DMEM and grown in serum-free DMEM supplemented with 50 ng/ml brain-derived neurotrophic factor (BDNF; Sigma Aldrich) for 7 ...
5C-5-4/25.1 Fuel Oil Tank Top Plating To simplify the requirement for fuel oil tank top structures not 32、contributing to hull girder bending strength. 5C-5-6/5.5 Stem Plating To consider the material factor in the net thickness of the plate stem, in line with CSR-H. 5C-5-A1/...
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Primary microglia were harvested from the mixed cultures by shaking flasks on an orbital shaker (200 rpm, 2 h, 37°C), centrifuging at 900 rpm for 5 min, and resuspending in DMEM-F12 supplemented with 10% FBS and plating as required by the experiment. At least 48 h are needed for ...
We have studied chrome-plating in standard and dilute electrolytes, making it possible to obtain hard chromium at a temperature of 45 to 48掳C and a current density from 50 to 130 A sol;dm 2 , and also wear-resistant chromium at 52 to 56掳C and a current density of 50 A/dm 2 . ...