Results Identification of the differentially expressed genes in three gonad types. To screen the differ- entially expressed genes during gonad reversal, 2D-electrophoresis was used to isolate the differentially expressed protein spots among ovary, ovotestis and testis. HE staining showed a typic ...
Electrophoresis 18, 2714–2723 (1997). 56. Schwede, T., Kopp, J., Guex, N. & Peitsch, M. C. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31, 3381–3385 (2003). 57. Zhao, M. et al. Mechanistic insights into the recycling machine of the SNARE ...
This clustering was chiefly confirmed by pulsed-field gel electrophoresis. One third of the bacilli were found to be psychrotolerant, which strongly supports the hypothesis of the existence of thermal ecotypes among B. thuringiensis. PCR screening was also performed to detect potential enterotoxin ...
Electrophoresis gel types and electrophoresis gel 专利名称：Electrophoresis gel types and electrophoresis gel 发明人：向出 雅一,熊沢 俊明,引地 一昌 申请号：JP特願平5-218033 申请日：19930810 公开号：JP特許第3344781号(P3344781)B2公开日：20021118 专利内容由知识产权出版社提供 摘要：Array 申请人：...
of the oligonucleotide probe, a Black Hole Quencher 1 moiety is attached to the 3′ end of the oligonucleotide probe, and the amplicon is detected by the oligonucleotide probe during real-time PCR. In another embodiment, the amplicon is detected by gel electrophoresis after the PCR is completed...
Protein concentration of samples was measured (Bio-Rad Protein Assay) and equal amount of protein was loaded for electrophoresis. Immunostaining analysis Neurons and glial cells After growing on glass coverslips, cells were washed three times with DPBS and fixed with 4% paraformaldehyde in PBS for ...
The amplified fragments were visualised by electrophoresis on 1.5% agarose gel stained with SYBR Safe. All S. aureus isolates were typed by pulsed-field gel electrophoresis (PFGE) using the modified protocol of McDougal et al. 17, as described in detail by Pereira et al. 18. Similarity was ...
The PCR products were analyzed by electrophoresis and the characteristic bands pattern on the electrophoresis gel would designate specific serotype for the bacterial isolate. We followed the methods described in previous works [32, 33]. For the data sets of certain serotypes (e.g. serotype II ...
Amplicon quality was determined by running a 5 ml aliquot of each product on a 1% agarose gel. Amplicons were only deemed suitable for sequencing if single high quality bands were clearly observed in the agarose electrophoresis gels. Before sequencing, PCR products were cleaned up by adding 4 ...
In accordance with the inventive methods, the expression level of the proteins encoded by the said genes is optionally detected by immunochemical means, such as, but not limited to, enzyme-linked immunosorbent assay (ELISA), immunofluorescent assay (IFA), immunoelectrophoresis, immunochromatographic as...