DNA extractioneDNAenvironmental DNAmacroorganismsmicroorganismsTwo-way interaction plots for soil chemical variables showing the effects of treatment and depth The interaction terms for (h) exchangeable acidity and (i) exchangeable aluminium were non-significant. Data are (dark blue) Permanent fallow; (...
Article: Comparison of three types of methods for the isolation of DNA from flours, biscuits and instant paps
The first step is sample preparation which begins with sample collection and DNA extraction from the samples of interest. DNA can be extracted using different methods including chemical lysis and mechanical methods. At first, the cells are lysed to release the DNA and then the cellular debris is ...
DNA Extraction and HPV Typing HPV infection outcomes were based on DNA of cervical and anal samples using a well-established polymerase chain reaction (PCR)–based protocol.34,35 Briefly, samples were processed in a biosafety cabinet in a laboratory that was physically separate from where the PCR...
Methods Data preparation We ensemble several germline mutation databases as the source of our datasets. Germline mutations are changes to DNA that individuals inherit from the egg and sperm cells during conception. ClinVar database (https://ftp.ncbi.nlm.nih.gov/pub/clinvar/, accessed 2022.6.24)...
of two types of repetitive DNAs in the genome of the grasshopper Eyprepocnemis plorans M Teruel1,3, FJ Ru´ız-Ruano1, JA Marchal2, A Sa´nchez2, J Cabrero1, JPM Camacho1 and F Perfectti1 Wide arrays of repetitive DNA sequences form an important part of eukaryotic genomes. ...
The Agricultural Research Centre of Mabegondo (Xunta de Galicia, A Coruña, Spain) conserves one of the most important collections of phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain, among them po
Cancer researchers explore new profiling methods DNA sequencing or denaturing high-performance liquid chromatography (dHPLC), qRT-PCR, or SYBR Green PCR and melting temperature analysis are used for point mutation profiling and discovery. They provide accuracy, sensitivity, reproducibility, and high prod...
MATERIALS AND METHODS Plant materials and DNA extraction The plant materials used in this experiment were two Gossypium A genome species (G. herbaceum, A1-97 and G. arboreum, A2-47), two D genome species (G. raimondii, D5-1 and G. trilobum, D8-1), one C genome species (G. stur...
Understanding the molecular and cellular processes involved in lung epithelial regeneration may fuel the development of therapeutic approaches for lung diseases. We combine mouse models allowing diphtheria toxin-mediated damage of specific epithelial cel