Plant Tissue Culture Media: Types, Constituents, Preparation and SelectionCornelius Onye Nichodemus
Tissue culture preparations of the myenteric plexus from the guinea pig taenia coli have been studied by electron microscopy. Three main cell types can be identified: neurons, enteric glial cells and fibroblasts. The ultrastructure of these cells resembles that of the same cells in situ. Neuronal ...
The development of animal cell culture techniques is due to the development of basic tissue culture media, which enables the working of a wide variety of cells under different conditions. In vitro culture of isolated cells from different animals has helped in the discovery of different functions an...
Tissue Culture:This technique involves the culturing ofplant cellsthat may be taken from different parts of a parent plant. The tissue is placed in a sterilized container and nurtured in a special medium until a mass of cells known as a callus is formed. The callus is then cultured in a h...
Collecting microbial culture and in vitro plant tissue Artificial propagation of plants and captive breeding of animals, with the possibility of reintroducing them back into the wild. Ex-situ conservation is an excellent way of averting the stresses on living organisms as a result of competition for...
Centromeres are the chromosomal domains required to ensure faithful transmission of the genome during cell division. They have a central role in preventing aneuploidy, by orchestrating the assembly of several components required for chromosome separation
Cell and tissue culture contamination with microbial contamination and other cell types not only affects the culture in question, but also compromises overall reproducibility of experimental results. Documentary: Cell Line Authentication Learn about cell line identification ...
They were also used as medicine in Chinese culture to remove the heat from the body. Straw mushrooms have mild, elegant tastes, and that makes them versatile in cooking. In Asian cuisine, they’re added to stir fry with noodles like chow mein, soup such as tom yum, or hot pot. You ...
HEPES is a zwitterionic organic buffer that is also used to maintain the physiological pH of cell culture media. When the cell culture system is very sensitive, the use of HEPES is recommended to increase the buffering capacity and stabilize the pH in the range of 7.2 to 7.6, regardless of...
A two-chamber perfusion bioreactor counteracts a significant amount of shear stress while allowing high nutrient transfer and removal of waste and/or valuable bio-products, e.g., protein, hormones, growth factors, and monoclonal antibodies without disturbing the cell culture. Cells and tissues, conta...