Class I biosafety cabinets are typically used to either enclose specific equipment like centrifuges or for procedures like aerating cultures that might potentially generate aerosols. Biosafety cabinets of this class are either ducted (connected to the building exhaust system) or unducted (recirculating ...
To remove excessive debris, the single cell suspension was passed through a 70-um filter into a 15 mL conical tube with 500 μl of high BSA buffer (ACSF with 1% FBS and 1% BSA) at the bottom to help cushion the cells during centrifugation at 100xg in a swinging bucket centrifuge for ...
After that time, the extract was centrifuged twice for 5 min in a 9.5 cm orbital radius centrifuge. The supernatant was transferred to a 25 mL volumetric flask, and made up to the mark with the same solvent. Finally, the extracts were stored at −20 ◦C prior to their analysis. ...