To remove excessive debris, the single cell suspension was passed through a 70-um filter into a 15 mL conical tube with 500 μl of high BSA buffer (ACSF with 1% FBS and 1% BSA) at the bottom to help cushion the cells during centrifugation at 100xg in a swinging bucket centrifuge for ...
1.An attenuated dengue virus (DENV) vaccine composition comprising one or more non-naturally-occurring attenuated DENVs selected from the group consisting of a mixture of attenuated serotype 1 dengue viruses (DENV1), a mixture of attenuated serotype 2 dengue viruses (DENV2), a mixture of attenu...
After that time, the extract was centrifuged twice for 5 min in a 9.5 cm orbital radius centrifuge. The supernatant was transferred to a 25 mL volumetric flask, and made up to the mark with the same solvent. Finally, the extracts were stored at −20 ◦C prior to their analysis. ...
(Centrifuge Mega Fuge 40 R, Thermo scientific, Langenselbold, Germany). The supernatant was collected, and the centrifugation and recovery of the supernatant was repeated. Finally, the samples were filtered through a 0.2 µm PTFE filter and they were deposited in amber vials for analysis by ...