Inflammatory caspases do not function in apoptosis and instead are involved in inflammatory cytokine signaling, danger signals and other types of cell death like pyroptosis. Thus, these caspases play a key role in innate immune response by inducing pyroptosis to stop intracellular replication of ...
Methods : HL60 cells and human umbilical vein endothelial cells (HUVECs) were incubated in the presence of the fluorescent inhibitor of caspases, FAM-VAD-FMK (20 mM, FLICA) for up to 48 h. Apoptosis was induced by Camptothecin (CPT, 0.15 μM) or by a mixture of tumour necrosis factor...
Apoptosis, the most common process of cell death, is a highly regulated, active mechanism mediated by the caspase cascade. It can be divided into internal (also known as mitochondrial) or external apoptotic pathways, depending on the inducing stimuli. Activation of the caspase cascade ultimately le...
Morales-Cano D, Calviño E, Rubio V, Herráez A, Sancho P, Tejedor MC et al (2013) Apoptosis induced by paclitaxel via Bcl-2, Bax and caspases 3 and 9 activation in NB4 human leukaemia cells is not modulated by ERK inhibition. Exp Toxicol Pathol 65(7–8):1101–1108 CASPubMedGoogle...
It causes proteolytic cleavage dependent activation of PARP as well as activator and executioner caspases of the intrinsic pathway. It also causes aberration in Bcl2/Bax, leading to the disruption of the mitochondrial membrane and production of ROS [38, 40, 41]. An in vitro study demonstrated ...
Hirata, H., et al., “Caspases Are Activated in a Branched Protease Cascade and Control Distinct Downstream Processes in Fas-induced Apoptosis”. J, Exp. Med., vol. 187, No. 4, pp. 587-600 (1998). Hizume, et al., “Tandem repeat DNA localizing on the proximal DAPI bands of chro...
of proinflammatory cytokines IL-1β and IL-18 but also to pyroptosis, whose main executor is gasdermin D (GSDMD), a member of the gasdermin protein family8,9. Upon proteolytic cleavage by inflammasome-activated caspases in the linker region connecting the N- and C-terminal GSDMD domains, the...
[66]Slee EA, Harte MT, Kluck RM, Wolf BB, Casiano CA, et al. (1999) Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J Cell Biol 144: 281–292. doi: 10.1083/jcb.144.2.281 ...
According to findings related to this study, this extract leads to either cell cycle arresting or apoptosis induction through inhibition of G2/M phase, increase of p21 and p53 levels and up-regulation of caspases and cytochrome c, respectively34. Given that chemotherapy and radiation lead ...
In the present study, we provide evidence that UV-irradiation of C6 cells leads to the activation of caspases, which in turn results in tau cleavage and NFT formation, a process that can be blocked with the cell-permeable pan-caspase inhibitor N-Benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoro...