Digital sorting of complex tissues for cell type-specific gene expression profiles. BMC Bioinform. 14, 89 (2013). Article Google Scholar Avila Cobos, F., Alquicira-Hernandez, J., Powell, J. E., Mestdagh, P. & De Preter, K. Benchmarking of cell type deconvolution pipelines for ...
Standard deconvolution algorithms require prior knowledge of the cell type frequencies within a tissue or their in vitro expression profiles. Furthermore, these algorithms tend to report biased estimations. Results Here, we describe a Digital Sorting Algorithm (DSA) for extracting cell-type specific ...
Digital sorting of complex tissues for cell type-specific gene expression profiles. BMC Bioinform. 14, 89 (2013). Article Google Scholar Avila Cobos, F., Alquicira-Hernandez, J., Powell, J. E., Mestdagh, P. & De Preter, K. Benchmarking of cell type deconvolution pipelines for ...
(dtangle39) and three methods included in the CellMix R package:19Digital Sorting Algorithm (DSA17) and two semi-supervised non-negative matrix factorization methods (ssKL and ssFrobenius18). Furthermore, five deconvolution methods that use scRNA-seq as reference have been evaluated: deconvSeq49,...
All fluorescence-activated cell sorting data were analyzed with FlowJo software v9.9.3 (FlowJo, LLC, Ashland, OR). Intracellular staining. Cells were blocked with Fc Block and stained with 10 nM MHC class II tetramer (I-Ab 2W1S-allophycocyanin [APC]) in sorter buffer for 1 h at room...
Although T-type channels undergo asparagine (N)-linked glycosylation32–35, the role of calnexin in the biogenesis and sorting of T-type channels is not understood. Here we show that calnexin binds to and modulates trafficking tIohnfeCardeadbv3yit.i2loecnahd, awinnegnsetholsoawtnottihnhacetrcc...
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The increase in the levels of miR-142-3p/-5p and miR-155 were detected also in fluorescence-activated cell sorting (FACS)-sorted β cells of pre-diabetic NOD mice (Figure 1B), confirming that the rise of these miRNAs occurs in insulin-secreting cells. In agreement with previous findings ...
We also drew attention to the methods of gene-edited cell selection showing that cell clones exerted biased functionality relative to polyclonal cells isolated by fluorescence-activated cell sorting (FACS). The design of sgRNA specific to different targets always resulted in an efficient gene ...
Sorting out the connections between the built environment and health: A conceptual framework for navigating pathways and planning healthy cities. J. Urban Health 2003, 80, 556–568. [CrossRef] [PubMed] 42. Giles-Corti, B.; Vernez-Moudon, A.; Reis, R.; Turrell, G.; Dannenberg, A.L.;...