Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper). Adjust final volume to a total of 10...
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following steps: adding the stabilizing agent, the preservative and the flame retardant into the deionized water in turn; and after evenly stirring, adding the rest components, namely the surfactant, the pour point depressant and the solvent, and stirring the mixture evenly to prepare the product....
Amino Acids Mixture Standard Solution, Type H Wako生产的氨基酸混合标准可用于氨基酸自动分析仪或高效液相色谱(HPLC)进行氨基酸成分分析或测序。 组成成分:(每种氨基酸浓度2.50μmol/ml,溶于约0.1 mol/L盐酸溶液) L-Aspartic Acid L -天冬氨酸 L-Threonine L -苏氨酸 L-Serine L -丝氨酸 L-Glutamic Acid L ...
[no. 15140148, Gibco] in Ham’s F-10 Nutrient Mixture [F-10] [no. 11550043, Gibco]). To obtain enough cells, the undigested tissue was digested once more, and the supernatant was collected. The supernatant obtained from the two digestions was centrifuged at 350 rpm for 10 min, ...
Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth. Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides. Xyloglucans and homogalacturonans are synthesized there actively ...
7. A method according to claim 6 for the making of a thin layer formed by at least two distinct monocrystalline semiconductor materials wherein the modulation of the composition of said layer is obtained by the controlled and checked modification, in said gas mixture, of the partial pressure an...
one for gene expression and the other one for DNA methylation. We evaluate the impact of several factors on the deconvolution results, including sample size, initial marker selection, endpoint selection, and number of cell types in the mixture. The simulation procedure is described in detail in ...
Because Sensei’s analytical solution includes necessary approximations (“Methods”, Eqs.7and10), we performed a simulation experiment to validate that Sensei accurately estimates the sample size for ideal beta-binomial distributions. We simulated 10,000 datasets using the beta-binomial model that Sens...
When copper hydroxide was used in a mixture with a wetting agent at a rate of 0.3 L ha−1 the measured deposit on the leaves was 68.28% when applied with SF nozzles. When the concentration of the wetting agent was doubled, the deposit of the solution reached 84.77% and was statistical...