M. Extracellular matrix binding properties of recombinant fibronectin type II-like modules of human 72-kDa gelatinase/type IV collagenase. High affinity binding to native type I collagen but not native type IV collagen. J Biol Chem 270, 11555–11566 (1995). 36. Overall, C. M. Molecular ...
Mouse IgG (Sigma, St Louis, MO), rabbit IgG (Endogen), and BP180 monoclonal antibody (LAD-1–123 antigen) was a generous gift from Dr Peter Marinkovich (Stanford University, Stanford, CA). Anti-human Von Willebrand Factor VIII (F-VIII) was from DAKO. Cell isolation and growth Cultures...
. Ganglia were incubated in type IV collagenase (Lorne Diagnostics, UK, 300 U/ml) for 3 hours at 37 °C at 5% CO2. Following several washes in the supplemented culture medium, ganglia were triturated with fire-polished Pasteur pipettes, and plated on poly-DL-ornithine (Sigma, UK)-...
Mouse antiInsulin Millipore Sigma Cat#I2018; RRID:AB_260137 Goat anti-rabbit-alexa fluor 488 Thermo Fisher Scientific Cat#A11008; RRID:AB_143165 Goat-anti-mouse-alexa fluor 546 Thermo Fisher Scientific Cat#A21123; RRID:AB_141592 Chemicals, peptides, and recombinant proteins Collagenase MilliporeSi...
containing 60 units/ml CLS4 collagenase (Worthington, Lakewood, N.J.) and 50 units/mL hyaluronidase (Sigma, St. Louis, Mo.). The dissociated chondrocytes (derived from the articular cartilage of a six year old individual) were washed in fresh HL-1 medium and briefly exposed to 0.25% EDTA...
(wt/vol) collagenase D (Sigma, cat. no. C0130-1G), 50 U/mL DNAse I (Sigma, cat. no. D4263-1VL) and 0.5% (vol/vol) FCS for 2–3 h at 37 °C. After enzymatic digestion, the cell suspensions were sieved through 70-µm cell strainers (Falcon) to remove tissue ...
(Sigma, D8161–5G) and 1 mg/mL (mouse) papain (Sigma, P4762-1G) added to the low-Ca2+-digestion solution; (2) with 0.5 mg/mL collagenase H (Sigma, C8051-1G), 0.7 mg/mL collagenase F (Sigma, C7926-1G) and 1 mg/mL soybean trypsin inhibitor II (Sigma, T9128-1G)...
The oocyte microinjection and two-electrode voltage clamp recording were performed as previously described40. The oocytes were collected from adult femaleX. laevis(purchased from Xenopus Express France, Vernassal, Haute-Loire, France) and pretreated by 1.5 mg/mL collagenase (Sigma-Aldrich Co., St...
Severe respiratory syncytial virus (RSV) infection is a major cause of morbidity and mortality in infants <2 years-old. Here we describe that high-fiber diet protects mice from RSV infection. This effect was dependent on intestinal microbiota and p
Cells were induced to differentiate in a differentiation medium consisting of: MEM, 10% FBS, 1% glutamine, 1 μM 2-butyl-cAMP (49.1 mg/100 mL media (Cat. #D-0627, Sigma Corp., St Louis, Mo.), and 2.5 mM bromo-deoxy-uridine (stock: 30.7 mg/10 mL media, 25 mL of above stock/...