M. Extracellular matrix binding properties of recombinant fibronectin type II-like modules of human 72-kDa gelatinase/type IV collagenase. High affinity binding to native type I collagen but not native type IV collagen. J Biol Chem 270, 11555–11566 (1995). 36. Overall, C. M. Molecular ...
M. Extracellular matrix binding properties of recombinant fibronectin type II-like modules of human 72-kDa gelatinase/type IV collagenase. High affinity binding to native type I collagen but not native type IV collagen. J Biol Chem 270, 11555–11566 (1995). 36. Overall, C. M. Molecular ...
containing 60 units/ml CLS4 collagenase (Worthington, Lakewood, N.J.) and 50 units/mL hyaluronidase (Sigma, St. Louis, Mo.). The dissociated chondrocytes (derived from the articular cartilage of a six year old individual) were washed in fresh HL-1 medium and briefly exposed to 0.25% EDTA...
Ethylmercurithiosalicylate (thimerosal), BSA (fraction V), and collagenase type IV were from Sigma-Aldrich (St. Louis, MO). DMEM was from BioWhittaker (Walkersville, MD). All other chemicals were of analytical grade. Animals Heterozygous srbI +/− mice on a 129(agouti)/C57BL/6 back...
For immunohistochemistry, sections were blocked in 3% H2O2, PBS containing 1% bovine serum albumin (A5611; Sigma-Aldrich, St. Louis, MO), 10% goat serum, avidin blocking solution, and biotin blocking solution (Avidin Blocking Kit; Vector Laboratories Inc., Burlingame, CA). For immunofluorescence...
The oocyte microinjection and two-electrode voltage clamp recording were performed as previously described40. The oocytes were collected from adult femaleX. laevis(purchased from Xenopus Express France, Vernassal, Haute-Loire, France) and pretreated by 1.5 mg/mL collagenase (Sigma-Aldrich Co., St...
Plasma DPP-IV level was measured using Mouse DPPIV ELISA kit (Sigma-Aldrich, USA). Plasma DPP4 activity was determined by the cleavage rate of 7-amino-4-methylcoumarin (AMC) from the synthetic substrate H-glycyl-prolyl-AMC (Gly-Pro AMC; Sigma), as described previously [21]. Pharmacological...
Cells. The human mast cell line, HMC-1 (kindly provided by J.H.Butterfield, Mayo Clinic, Rochester, Minn., USA) [26] was cultured in Iscove's medium supplemented with 10 % heat-inactivated calf serum (Hyclone, Logan, Utah, USA) and 1.2mmol/1 alphathioglycerol (Sigma, St.Louis, Mo....
Mouse IgG (Sigma, St Louis, MO), rabbit IgG (Endogen), and BP180 monoclonal antibody (LAD-1–123 antigen) was a generous gift from Dr Peter Marinkovich (Stanford University, Stanford, CA). Anti-human Von Willebrand Factor VIII (F-VIII) was from DAKO. Cell isolation and growth Cultures...
Of these, membrane-type 1 matrix metalloproteinase (MT1-MMP) functions as a potent peri-cellular collagenase capable of degrading not only soft type I and type II collagen, but also components of the basement membrane such as laminin and type IV collagen (Hotary et al., 2003, Hotary et ...