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Sapranauskas, et al., " Novel Subtype of Type IIs Restriction Enzymes: Bfii Endonuclease Exhibits Similarities to the Edta-resistant Nuclease nuc of Salmonella typhimurium, " J. Biol. Chem. 275:30878-30885 (2000).Novel subtype of type IIs restriction enzymes. BfiI endonuclease exhibits ...
Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling: a one-pot DNA shuffling method based on type IIS restriction enzymes. PLoS One, 2009, 4(5): e5553. Gilbert C, Ellis T. Biological Engineered Living Materials: Growing Functional Materials with Genetically Programmable Propertie...
Figure 1. Overview of the various cloning strategies possible when using GeneArt Type IIs Assembly Kits.(A) Diagram of the seamless assembly of two fragments using a combination of Type IIs restriction enzymes and ligase. The fragments and the corresponding sequences at their e...
Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling: a one-pot DNA shuffling method based on type IIS restriction enzymes. PLoS One, 2009, 4(5): e5553. Gilbert C, Ellis T. Biological Engineered Living Materials: Growing Functional Materials with Genetically Programmable Propertie...
6、ndzia r, marillonnet s (2009) golden gate shuffling: a one-pot dna shuffling method based on type iis restrictionenzymes.plosone4(5):e5553.doi:10.1371/journal.pone.0005553editor:jeanpeccoud,virginiatech,unitedstatesofamericareceivedfebruary3,2009;acceptedapril19,2009;publishedmay14,2009copyrig...
However, restriction endonucleases can be influenced by the sequences flanking the recognition site (2), and the type IIs enzymes may also be affected by the sequence throughout the length of DNA between recognition and cleavage sites. Consequently, wherever practicable (Fig. 1, b–d), the ...
Figure 1. Overview of the various cloning strategies possible when using GeneArt Type IIs Assembly Kits.(A) Diagram of the seamless assembly of two fragments using a combination of Type IIs restriction enzymes and ligase. The fragments and the corresponding sequences at their ends are shown in ...
摘要: A new type-IIS restriction enzyme, Bst71I, with the specificity 5'-GCAGC(N)8/3'-CGTCG(N)12 was isolated from Bacillus stearothermophilus (Promega No. 71). This enzyme is an isoschizomer of BbvI with somewhat improved characteristics for use by molecular biologists....
This protocol is based on the use of type IIs restriction enzymes and is performed by simply subjecting a mix of 10 undigested input plasmids (nine insert plasmids and the acceptor vector) to a restriction-ligation and transforming the resulting mix in competent cells. The efficiency of this ...