目前已知的CRISPR家族被分为Type I至Type VI,其中靶向RNA的家族是Type III和Type VI。Type VI家族的效应器是由多结构域组成的单一蛋白,负责crRNA成熟和target RNA切割。相反,Type III家族效应器则由多个蛋白组成,进行RNA干扰。由于单一蛋白的简并性,因此,科研团队往往把更多的关注放在了Type VI家族,并希望借此进行...
Although Type III CRISPR-Cas systems are among the most common CRISPR-Cas systems, they are also some of the least investigated ones, mostly due to the complexity of their action compared to other CRISPR-Cas system types. Type III effector complexes specifically recognize and cleave RNA molecules...
The article reports on the results of three studies which show the structural characterization of the complexes of type III clustered, regularly interspaced short palindromic repeats-(CRISPR)-associated proteins from Sulfolobus solfataricusa.doi:10.1038/nrmicro3166Christina Tobin Kåhrström...
该论文首次阐述了在Nla Type I-C系统中,Cascade结合靶标DNA之后,Cas3如何通过与Cas8亚基“握手”的方式被招募并激活的分子机制;此外该项研究筛选到AcrIC8和AcrIC9是Nla Cascade-Cas3系统的Anti CRISPR蛋白,在体内外均能强烈导致Typ...
Type III CRISPR-Cas loci encode some of the most abundant, yet complex, immune systems of prokaryotes. They are composed of a Cas10 complex that uses an RNA guide to recognize transcripts from bacteriophage and plasmid invaders. Target recognition triggers three activities within this complex: ssD...
In the type III CRISPR–Cas immune response of prokaryotes, infection triggers the production of cyclic oligoadenylates that bind and activate proteins that contain a CARF domain1,2. Many type III loci are associated with proteins in which the
differentpathogensthatthreatenthehost.CRISPR- Casimmunesystemsprotectprokaryotesfromviral andplasmidinfectionutilizingsmallCRISPRRNAs thatarecomplementarytotheinvader’sgenome andspecifythetargetsofRNA-guidedCasnucle- ases.TypeIIICRISPR-Casimmunityrequirestarget ...
Here, we present the discovery of a type III CRISPR-Cas inhibitor, AcrIIIB1, encoded by the Sulfolobus virus SIRV2. AcrIIIB1 exclusively inhibits CRISPR-Cas subtype III-B immunity mediated by the RNase activity of the accessory protein Csx1. AcrIIIB1 does not appear to bind Csx1 but, ...
Prokaryotes have evolved diverse defense strategies against viral infection, such as foreign nucleic acid degradation by CRISPR-Cas systems and DNA/RNA synthesis inhibition via nucleotide pool depletion. Here, we report an antiviral mechanism of type III CRISPR-Cas-regulated ATP depletion, where ATP is...
第二项工作是关于Desulfonema magnum type III-E CRISPR-Cas 系统特异性RNA核酸酶的功能调控。 CRISPR-Cas系统通过序列导向的核酸降解,为宿主原核生物提供适应性免疫,是生物医学研究和基因治疗的强大基因工具。新发现的type III-E CRISPR...