Over the billions of years that bacteria have been around, they have evolved several sophisticated protein secretion nanomachines to deliver toxins, hydrolytic enzymes, and effector proteins into their environments. Of these, the type II secretion system (T2SS) is used by Gram-negative bacteria to ...
work on this system and argue that intrinsically disordered regions and proteindynamicsare central for assembly, exo-protein recognition, and secretion competence of the T2SS. The central role of intrinsic disorder-order transitions in these processes may be a particular feature of type II secreti...
Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen. We show that 7 proteins form a ~2.4 MDa...
The T9SS cargo proteins share a conserved C-terminal domain (CTD) composed of about 80 amino acid residues as a secretion signal for the T9SS. Sequence analysis of CTDs have revealed that T9SS CTDs belong to either protein domain family TIGR04183 (type A CTDs) or TIGR04131 (type B ...
四型分泌系统 (type IV secretion system, T4SS) 是一种在革兰氏阴性菌 、革兰氏阳性菌和古细菌中均广泛存在的多功能、多组分的跨膜通道结构,将DNA,蛋白质或其他大分子递送至靶细菌或真核细胞。与细菌中已发现的其它分泌系统不同,T4SS形成的管道既能通过蛋白质又能通过DNA,因此T4SS能够介导细菌接合转移、DNA释...
Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm forma...
Type VII protein secretion (T7SS) is a specialised system for excreting extracellular proteins across bacterial cell membranes and has been associated with virulence in Staphylococcus aureus. The genetic diversity of the ess locus, which encodes the T7SS
The type III secretion system (T3SS) is a protein secretion machine found in Gram-negative patho- genic bacteria. This multimeric heteroprotein structure is characterized by a long passageway that is 2–3 nm in internal diameter, termed the needle [4]. Given the diameter of a typical ...
Conservative analysis of type III secretion system chaperone protein sequence and prediction of new chaperone致病菌III型分泌系统分子伴侣序列保守性分析及新伴侣的预测Ⅲ型分泌系统分子伴侣毒力因子Many Gram negative pathogen utilize type III secretion system (TTSS) to inject toxins into the cytosol of ...
Goosens VJ, Walker KT, Aragon SM, et al. Komagataeibacter Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria. ACS Synth Biol. 2021, 10(12): 3422-3434.