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The remaining tissue was then placed in PBS and finely diced before being digested in type I collagenase (3 mg/mL Sigma–Aldrich, St. Louis, MO, USA) for 2.5 h at 37 °C on a rocking bed set at 200 rpm. A single-cell suspension was yielded using a 70-μm cell strainer...
COLLAGEN TYPE I (BOV INNER SKIN) - FITC CONJUGATED生产厂家 全球有 5家供应商COLLAGEN TYPE I (BOV INNER SKIN) - FITC CONJUGATED国内生产厂家 供应商联系电话电子邮件国家产品数优势度 Creative Enzymesinfo@creative-enzymes.com美国605758 SIGMAALDRICH美国677387 ...
Collagenase IV Gibco Cat# 17104019 Dispase II Gibco Cat# 17105041 Liberase Roche Cat# 5401020001 DNase I Roche Cat# 10104159001 Type I collagen solution from rat tail Sigma-Aldrich Cat# C3867 Critical Commercial Assays Direct-zol RNA Kit Zymo Research Cat# R2050 Reverse Transcription Kit Applied ...
Tissue pieces were placed in 150 ml of EMEM that contained collagenase (0.75 mg/ml, Collagenase B, #11088831001, Roche) and DNase I (0.075 mg/ml, #10104159001, Roche) and were incubated at 37 °C for 30 min with stirring. The dispersed mesenchymal cells were collected by ...
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Briefly, mice were treated with APAP and 6, 12, and 24 h after treatment mice were anesthetized and submitted to liver perfusion with collagenase (Sigma-Aldrich, C2139) through the portal vein. After the perfusion, the liver was transferred to a sterile glass container and dissociated with ...
(ThermoFisher Scientific) in 1X PBS for 15 min at 37 °C and permeabilized using 0.1% of Triton™ X-100 (Sigma Aldrich) diluted in 1X PBS. Samples were stained with Alexa Fluor™ 488 Phalloidin (1:400, ThermoFisher Scientific) to visualize F-actin and counterstained with 4′,6-...