BERTHOLD 型号:Crystal CsI 40x50 mm 代号LB 6739-01 CsI 40x50 mm JAHN JLF II WAGO 787-881 PROXITRON ST040 M12*1 HYDAC HDA4445-A-016-000 PALL HC8314FKS16H EUCHNER 98941 ROLAND IE20-30GS PN:S0003322 MTS RHM0110MP021S1G8101 ELCIS I/115-1024-18285-BZ-N-CW-R-03 MAXIMATOR S25-02 ...
Human DNase II constructs with a C-terminal FLAG sequence were introduced into DNase-II-deficient HEK293FT via calcium phosphate transfection. After 36 h, the cells were subjected to cytosolic lysis with a buffer containing 50 mM Tris HCl, pH 7.4, 150 mM NaCl, 50 mM EDTA, 1% TRI...
Cells were then washed once with PBS, lysed with 300 μL lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris-HCl pH 7.2, 1 mM Na3VO4, 10 mM β-glycerophosphate, 50 μg/mL PMSF with SIGMA FAST protease inhibitor) and placed on ice for 20 min and centrifuged at 12,000 × g for...
66,021 Cytochrome b-245 beta chain Cybb Forward: CGAAGACAACTGGACAGGAACCTTAC Empty Cell Reverse: TTGAACAGATGTGCAATGGTGTGAATG 24,534 Lactate dehydrogenase B Ldhb Forward: CAATGACAGCGAGAACTGGAAGGAG Empty Cell Reverse: GATTCGATGAGGTCAGCCACACTTAG 498,638 Macrophage scavenger receptor 1 Msr1 Forward...
6. Water Level Control in a Tank The water level control in a tank, illustrated in Figure 4, is a classical benchmark control problem and has been considered as a study case to improve the performance of FLC controllers and to compare FLC T1 and FLC T2, and other works with this plant...
2. Experimental Apparatus The wave tank test was carried out using regular waves, though real sea waves are irregular, because it is difficult to completely absorb the reflected wave with the wave generator in the case of irregular waves. Besides, it seems that the test with regular waves is...
(引物序列如表 1 所示),上述基因扩增体系 为 40 μL:基因组模板 DNA 2 μL,2×T8 High-Fidelity Master Mix 20 μL,上下游扩增引物各 2 μL,加双蒸水 补足 40 μL.扩增反应程序:98 ℃预变性 2 min,95 ℃ 10 s,退火温度(recA 60 ℃;rpoB 65 ℃;gyrB 66 ℃) 30 s,72 ℃ 2 min,35 个...