product information Background TYLCV C2 (Tomato yellow leaf curl virus coat protein C2) accumulates in tomato leaves during infection. This protein was detected in the phloem of associated cells. TYLCV virus attacts tomato cultures worldwide a
PURPOSE: A method for preparing polyclonal antibody for TYLCV-C2-6X His fusion protein is provided to develop a virus diagnosis kit.;CONSTITUTION: A method for preparing TYLCV(tomato yellow leaf curl virus)-C2-6X His fusion protein comprises: a step of cloning TYLCV-C2 gene to pET28a vector...
PURPOSE: A method for preparing polyclonal antibody for TYLCV-C2-6X His fusion protein is provided to develop a virus diagnosis kit.;CONSTITUTION: A method for preparing TYLCV(tomato yellow leaf curl virus)-C2-6X His fusion protein comprises: a step of cloning TYLCV-C2 gene to pET28a vector...
通过酵母双杂交实验,双分子荧光互补实验,共定位,确定了 C7分别与TYLCV编码的V2和C2互作.C7单独定位到细胞核和细胞质中,但是与V2共定位在细胞质中,与C2共定位在细胞核中.进一步实验发现C7可以抑制GFP诱导的转录后基因沉默(Post transcriptional gene silencing,PTGS),利用马铃薯X病毒(Potato virus X,PVX)载体过表达...
刘佰明徐维红刘晓琳霍建飞白鹏华王勇
结果表明,通过RNAi技术干扰TYLCV V1和C1基因可以延缓病毒症状的发生,提高番茄植株对TYLCV的抗性.田间试验结果发现,以TYLCV V1和C1为靶标的RNAi株系RNAi-CP-2和RNAi-Rep-12,不仅可以干扰病毒V1,C1的表达,而且可以干扰TYLCV其他4个基因V2,C2,C3和C4,表明RNAi技术可以在靶标基因附近传递,影响相邻其他基因的表达.本...
In this study, PCR technique was used to amplify partial sequences (670 bp) of tomato yellow leaf curl virus (TYLCV) genome, specifically spanning the trans-activator protein (C2), replication enhancer protein (C3) genes, as well as partial parts of replication...
In order to generate engineered resistance,the plant expression vectors SUC2-GroEL and pBI121-AC1-AC2 were co-transformed into tomato by agrobacteriummediated genetic transformation method,and nineteen kanamycin resistant regenerated tomato plants were obtained,including five plants of SUC2-GroEL vector,...