Many techniques have benefitted from temporal focusing, including scanless imaging, super-resolution imaging, photolithography, uncaging of caged neurotransmitters and control of neuronal activity via optogenetics. This is a preview of subscription content, access via your institution Access options Access...
. For each chirp configuration, the ETL in detection path was used to obtain a 200-µm deep stack (step size: 1 µm) centered at the nominal focal plane of the illumination objective. Supplementary Fig.10ashows the axial position of the fluorescent intensity peak as a function of the...
Third, whether single-cell two-photon optogenetics can be efficiently applied across the various cell types that are engaged during sensory stimulation and that differ in morphology, biophysical properties, and cortical depth is unclear. Here we developed an experimental approach to address all of ...
Benefits of using two-photon excitation include specifically exciting fluorophores only in the actual focal volume and reduced phototoxicity and photobleaching. Two photon microscopy enables excitation of fluorophores deeper in tissue, as the light used is a longer wavelength so can penetrate deeper into...
OPEN SUBJECT AREAS: OPTICAL IMAGING OPTOGENETICS BIOLOGICAL FLUORESCENCE TECHNICAL REPORT Received 13 May 2013 Accepted 21 June 2013 Published 19 July 2013 Correspondence and requests for materials should be addressed to T.K. (tknopfel@knopfel- lab.net) * The authors contributed equally to this ...
Light: Science & Applications (2016) 5, e16007; doi:10.1038/lsa.2016.7; published online 1 January 2016 Keywords: angiography; eye model; functional calcium imaging; in vivo; optogenetics; two-photon microscopy INTRODUCTION Non-invasive fluorescence fundus imaging is an important tool for in vivo ...
By spatially dispersing the optical pulse with the grating, the laser's pulse width is broadened temporally, and the peak power is reduced everywhere apart from at the focal plane of the microscope, thus ensuring that multiphoton excitation is confined to this plane. This technique has been ...