三杂交系统(three-binding hybrid system)用于分析蛋白和RNA间的相互作用。Senguptaxi等首先报道了应用三杂技系统分析金属调节蛋白(iron regulatory protein-1,IRP1)与金属反应元件(iron response element,IRE)RNA序列,以及HIV转录激活蛋白(trans-activator protein, Tat)与HIV转录激活反应元件(HIV trans-activation response...
hybrid system has been used to identify interacting proteins of the pore forming subunit of the cardiac KATPchannel, Kir6.2 using a truncated version of the protein (residues 169–354) as a bait[51]. Moreover, a yeast two-hybrid system optimized formembrane protein interactions(MbYTH) has ...
【答案】:酵母特异启动子结合的转录因子蛋白由两个相对独立的结构域(DNA结合结构域和转录激活结构域)组成,如果二者分开则不能表现出对下游基因表达的激活作用。如果两个结构域分别融合了具有配对相互作用的两种蛋白质分子后,就可以依靠所融合的蛋白质分子之间的相互作用而恢复对下游基因的表达激活作用。 收起 为你推荐...
生物化学词汇英汉对... ... yeast knockout project 酵母基因剔除yeast two-hybrid-system酵母双杂交系统zinc finger 锌指结构 ... www.biomsn.com|基于6个网页 2. 双杂交技术 ...p)的概念与应用。蛋白质相互作用的研究技术:酵 母双杂交技术(yeast two-hybrid-system)的概念、基本原理与应用。 1. 周… ...
Yeast mating type switching and yeast two-hybrid system 泛基诺技术总监 胡恩泛 读者或和科研人员在接触酵母双杂交系统时,总会看到酵母细胞表型(基因型)的标记,如Mata或Matα,后面还紧跟一联串的小写标记基因符号如ura3,his3,leu2,trp1,ade2,can1-100。在查阅文献时,又苦于缺乏清晰而又系统的诠释资料,这对于...
The two-hybrid system (THS), first introduced by Fields and Song (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest...
Twitter Google Share on Facebook yeast (redirected fromyeast two-hybrid system) Thesaurus Medical Acronyms Encyclopedia yeast (yēst) n. 1. a.Any of various unicellular fungi of the genusSaccharomyces,especiallyS. cerevisiae,reproducing asexually by budding or sexually through the production of ascospo...
翻译结果3复制译文编辑译文朗读译文返回顶部 Yeast two-hybrid system 翻译结果4复制译文编辑译文朗读译文返回顶部 Yeast two-hybrid 翻译结果5复制译文编辑译文朗读译文返回顶部 Yeast double hybrid 相关内容 a我很伤心但是装着镇静 But I very sad am installing calmly [translate] a另一些人认为应该控制私人购买汽车...
系统标签: yeast clontech hybrid manual user 酵母 Matchmaker™GoldYeastTwo-HybridSystemUserManualPT4084-1(PR033493)Cat.No.630489Published30March2010UnitedStates/Canada800.662.2566AsiaPacific+1.650.919.7300Europe+33.(0)1.3904.6880Japan+81.(0)77.543.6116ClontechLaboratories,Inc.ATakaraBioCompany1290Te...
the appropriate two-hybrid system needs to be chosen at least in part based on the sub-cellular localization of the proteins of interest. For example, standard Y2H requires translocation of interacting proteins into the nucleus[35], which can lead to false negative errors if either interactor is...