Next, we generated a null allele by replacing the SML1 ORF with a HIS3 marker. Like sml1-1 strains, the sml1Δ does not exhibit a growth defect. Both alleles completely rescue the lethality of mec1-1 or mec1Δ strains (Figure 2A). Thus, it is likely that the loss of the TATA ...
Expression plasmids pVITRO1-MCS are developed mainly for in vitro studies. pVITRO1-MCS allow the ubiquitous and constitutive co-expression of two genes of interest. pVITRO1-MCS plasmids can be stably transfected in mammalian cells and the genes of intere
Recently, a bone injuring model by using zebrafish has been reported, indicating that expressions of the fin regeneration marker genes delay during fracture healing compared with fin regeneration after amputation (Sousa et al., 2012). Here, we focus on the cellular behavior of osteoblasts and ...
Yes-associated protein is an independent prognostic marker in hepatocellular carcinoma. Cancer 115, 4576–4585 (2009). Article CAS PubMed Google Scholar Zhao, B. et al. Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. Genes ...
Lignin is a complex polymer deposited in the cell wall of specialised plant cells, where it provides essential cellular functions. Plants coordinate timing, location, abundance and composition of lignin deposition in response to endogenous and exogenous
(plant GOSlim) of these genes again identified signal transduction, stress responses and cell death as enriched upregulated biological processes whereas photosynthesis as well as growth and development were downregulated (Supplementary Table3). The observation that many upregulated XXM DEGs are involved...
Expansion of these genes in switchgrass was achieved through both fragment and tandem duplication. PvTCS members are relatively conservative in the evolutionary process, but the gene structure varies significantly. Various cis-acting elements, varying in types and amounts, are present in the promoter ...
The development of a genetic linkage map would be a significant breakthrough, either to map candidate genes, to search for QTL of agronomical traits, or to make marker-assisted selection. Such analysis requires working on cultivated material, either in terms of genetic background or ploidy level...
For example, genes that determine entry into the host or that modulate host behavior to regulate exposure to the spores may have a role in horizontal, but not vertical, infection processes, while genes responsible for within host transmission and growth might affect both types of infections. The...
‘two-hybrid’), the function of the factor can be restored to transcribe one or moreselectable markeror reporter genes that then select for the interaction event (Figure 2). Since its first introduction nearly 20 years ago[14], many improvements and enhancements have been developed, and these...