We used the doxycycline (Dox)-inducible, Tet-On system to develop transgenic zebrafish for inducible, cell specific control of gene expression in the ultraviolet (UV) cone photoreceptors. Two constructs containing the reverse tetracycline-controlled transcriptional transactivator (rtTA) gene driven by ...
So we were sent off with a round of Doxycycline for the cough, and aharnessfor Bowpi is already on the way. I definitely feel some guilt, wondering if this all could have been prevented if I had just been walking Bowpi on a harness to begin with. Like pretty mucheveryBasenji I’ve...
Doxycycline doxycycline,cyclosporine, minocycline, erythromycin and erythromycin Acyclovir - acyclovirisoproterenol - etoposide etoposide - panting Bucinnazine - AP-237pethidine - dolatin compound guaiacol potassium sulfonate - phenargan mixture Lin leflunomid - Arava -ferrous succinate ferrous succinate - li...
Doxycycline hyclate Tocris Cat#4090 Puromycin dihydrochloride Tocris Cat#4089 Activin A R&D Systems Cat#338-AC Greiner Bio-one Cell View glass plates Greiner Bio-one Cat#627965 4% Paraformaldehyde in PBS Thermo Fisher Scientific Cat#R37814 Triton X-100 Sigma Cat#T8787 PBS w/o CaMg Sigma Cat...
Two most common causes of urethritis? C trachomatisN gonorrhea How do you diagnose urethritis? NAAT How do you treat urethritis negative for gonococci? (2) AzithromycinDoxycycline Symptoms of epididymitis? (2) DysuriaUnilateral scrotal pain Most common pathogen that causes epididymitis in men <...
Terminally differentiated cells can be generated by lineage reprogramming, which is, however, hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking
doxycycline (dox)-inducible lentiviral vectors and E15.5 mouse embryonic fibroblasts were infected with one or more lentiviruses and cultured in a SC culture medium supplemented with Neuregulin-1 (NRG1) and forskolin (Fsk) (Fig. 1a)20. At first, we realized that primary cultures of embryonic ...
ΔOTC/HeLa-TetOn cells with the stable expression of YFP-Parkin were treated with doxycycline (DOX, 1 μg/mL) for 72 h to induce mitochondrial ΔOTC expression. After removal of DOX, cells were treated with the indicated concentration of drugs for 8 h. Asterisk, non-specific. (E) T0466...
1.2 mM sodium butyrate (NaB) (Aladdin Industrial Corporation) and 1 μg/mL doxycycline (Dox) (Aladdin Industrial Corporation) were used to promote the lytic replication of KSHV18,19. The iSLK.219 cells grown to 80% confluence within 96-well plates were accurately added with the ...
(5 × 15 cm dishes) in three biological replicates were induced with 1 μg/ml doxycycline (MP Biomedicals) for 24 h. After induction, cells were washed with 0.1 mM MgCl2, 0.1 mM CaCl2in PBS and harvested in 1 mM EDTA-PBS. Cells were pelleted by centrifugation 400...