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Based for FET system, Rawat et al. introduced a biosensor using two-dimensional MoS2for the detection and quantification of glutathione (GSH), an important biomarker for various diseases, including cancers [63]. The sensor employed glutathione-S-transferase (GST) immobilized on the MoS2surface, tak...
Accordingly, similar chemical shifts for the region of RbFox interacting with UGC were observed in the two complexes, indicating that the UGC nucleotides in both structures maintain the same contacts within the RNA-binding cleft of RbFox. In contrast, the G- > A substitution at position 6...
The G/A151D and W271L mutation significantly reduced carboxylesterase activity in 87.5% and 100% cases, respectively, and at the same time conferred OP hydrolase activities in 62.5% and 87.5% cases, respectively. Thus, the change at position 271 is more effective to influence substrate ...
After two washes with Wash Buffer (30 mM MgCl2, 25 mM Tris pH 7.5, 40 mM NaCl), denatured samples were analyzed by western blot using His (Invitrogen, Clone name: HIS.H8; Catalogue number: MA1-21315; 1:1000 dilution) and GST (Invitrogen, Clone name: 8–326; Catalogue number:...
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The bound RNAs were eluted and resolved on a polyacrylamide gel. Efficient binding of GST-HUA1, but not GST-αCP1, to all three RNA segments was detected (Figure 7 and data not shown). To determine whether HUA1 was specific for these regions in the AG pre-mRNA, we tested a probe ...
First of all, can you try run the same pipeline with GST_DEBUG=5 in front of it, and send us the logs? It might provide us more information than "Internal data stream error". Also, just to isolate the problem, have you tried to run the pipeline with videotests...
(a). The dome region is marked by pink lines.cQuantification of the intensity ratio of peak and base in the plots. The number of cells used for quantification is shown at the bottom. Data are presented as mean values ± SD with individual points shown. The comparison was performed...
The related coding sequences were re-cloned from the previously made constructs of the GST-HEV P and GST-VP837. The AstV P domain-encoding sequences were synthesized chemically via Genscript (Piscataway, NJ). A plasmid containing the pQE30 and AstV P-12G-HEV P coding sequences (...