The total-vaccine and standard-range samples were diluted in ELISA buffer comprising a desorption buffer, in 2-fold serial dilutions in a range included, for example, between 1/8 and 1/25 600. A 96-well plate coated with the primary monoclonal antibody was incubated for 12 h at 5° C....
The new measure serves a two - fold purpose of providing employment and stimulating the economy. 这项新措施一举两得,既提供了就业机会,又刺激了经济发展. 互联网 The in vitro antimicrobial activity was evaluated by the serial two - fold agar dilution method. ...
The black triangles indicate two-fold serial dilutions of the amount of the chromatin sample used in each PCR reaction. More volumes of the indicated template were in the PCR reactions on the left as indicated by the thicker part of each triangle. (D) The anti-Cp190 ChIP of y2ct6; CP...
For the kinetic measurements of SARS-CoV-2 VOCs’ RBDs to EH3 and EH8, either of the IgGs (∼250-350 response unit, RU) was first immobilized on a protein A chip (Cytiva) and 2-fold serial dilutions of the RBDs were then injected with concentrations ranging from 6.25 to 200 nM...
The checkerboard titration assay was conducted to determine the optimal concentrations of protein coating and serum samples. Recombinant SVA 3AB proteins from both expression systems were individually analyzed with 2-fold serial dilutions to 2, 1, 0.5, 0.25, and 0 µg/ml. Similarly, positive...
f Venn diagram of 1.5-fold change in IFN-γ-inducible genes. Diagram shows microarray analysis of upregulated genes following IFN-γ stimulation (100 ng/mL for 8 h) in U3A cells transfected with WT, H629Y or R274G STAT1. g U3A cells transfected as indicated with WT, H629Y or ...
(from 4 mg/ml to 0.0625 mg/ml, serial two-fold dilutions) were prepared for this experiment in the OdhA buffer (20 mM Hepes pH 7.5, 500 mM NaCl) and loaded into analytical ultracentrifugation cells. During the run, SV was followed by measuring absorbance at 280 nm for ...
(Supplementary Fig. 3B). Deletion of residues 440–460 from full-length BubR1 reduced its interaction with Bub1 almost a 100-fold based on quantitative western blot of immunopurifications of BubR1 complexes from nocodazole-arrested cells. Bub3 binding was not affected showing that residues 440–...
Test sera were heat-inactivated at 56°C for 30 min and diluted 1:5 and in serial two fold dilutions (up to 1:2,560). The Nakayama strain (heterologous SA-14-14 strain) was diluted in phosphate buffer saline containing 5% fe- tal calf serum and 5% guinea ...
Six two-fold serial dilutions each with three replicates were used. Both extracts, at the highest concentration 150 mg/ml (15%), induced 100% failure of hatching of the treated eggs. The concentrations of PE and EE that induced 50% inhibition of the hatchability (IC50) were 1.71 and ...