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Fig. 3: Long time-lapse two-color live-cell STED imaging of HeLa cells. a Schematic illustration of the three-step procedure employed to label the plasma membrane and ER. b Two-color STED image of the plasma membrane and ER of HeLa cells. Images from both channels are shown. Scale bars...
blocks containing pixels of a foreground color and pixels of a background color or two-color blocks, and blocks containing pixels with more than two colors or multicolor blocks, and coding, in the image data of the single-color blocks and the two-color blocks, by command code words and dat...
Choose the image or photo you’d like to use as the background and create a new file with the correct dimensions. Set up your background. Select the Layers menu and click the lock icon to unlock and set the background color to transparent. Now, drag and drop your background image into...
1d). In contrast to the unspecific mitochondrial staining of ATTO647N in living cells6, no unspecific binding to intracellular membranes and only very little background due to endocytosis of the dyes was observed (Supplementary Fig. 5). Figure 1: STED nanoscopy of dynamic interactions between ER...
We know women of color are more often under-mentored and under-sponsored than their White counterparts. How are you both working to change that at McKinsey? Gayatri:Anytime I have a new opportunity—like a speaking engagement, for instance—I offer it to a minority woman before...
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