TWO STAGE CLEAVAGE OF CUMENE HYDROPEROXIDEPURPOSE: To prepare phenol and acetone with the improved two-stage method which is comprised of decomposing cumene hydroperoxide(CHP) in a first stage and hydrogenating the products of the first stage in a second stage.DECARIA ANTHONY J...
Therefore, it was concluded that there was neither gametic selection against genome imbalance nor zygotic selection from fertilization until the first cleavage metaphase. However, 9.1–10.8% of embryos were arrested at the two-cell stage, and karyotypes of these embryos were confirmed as 22(2,10...
We separated the blastomeres of 1,252 two-cell embryos, preserving 1,210 of the pairs. Two classes of monozygotic twins became apparent at the blastocyst stage: 27% formed a functional epiblast in both members (concordant), and 73% did so in only one member of the pair (discordant) - a...
Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers. BACKGROUND: embryo biopsy is performed for preimplantation genetic diagnosis (). The impact of 1- or 2-cell removal at cleavage-stage on future embryo and .....
Alternatively, we show that by using an engineered MMR inhibitor and editing during the two-cell stage of development (PEmbryo), we can achieve high installation efficiencies of small programmed edits (primarily substitutions) without the use of complementary-strand nicks. We thus establish MMR as ...
Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequ
MicroRNAs (miRNAs), a subset of non-coding RNAs, are ~ 22 nucleotides-long endogenously-produced short RNA molecules that either manipulate mRNA cleavage post-transcriptionally or repress translation of the target mRNA [27]. miRNAs are involved in various biological processes such as cell divisi...
Two-cell embryos that were newly formed at 30 h and underwent the next cleavage within the first 2 days of IVC (30 + group) had a higher blastocyst rate (74.8 ± 7.0%) and greater cell numbers (40.6 ± 2.6) than those not showing a second division during this period (30– group; ...
Sequence analysis of the cloned 610-bp fragment confirmed that it is the PAF receptor. Northern blot analysis also confirmed the expression of the PAF receptor in the CFW mouse preimplantation two-cell-stage embryo. PAF treatment of the two-cell-stage CFW mouse embryo resulted in a fourfold ...
Here, we describe two-cell homologous recombination (2C-HR)-CRISPR, a highly efficient gene-editing method based on introducing CRISPR reagents into embryos at the two-cell stage, which takes advantage of the open chromatin structure and the likely increase in homologous-recombination efficiency ...