Background:Blastomere multinucleation (MN), defined as the presence of more than one nucleus per cell, in the cleavage-stage embryo, is a well-documented phenomenon1. This nuclear anomaly has become more visible with the introduction of time-lapse incubation and an understanding of the potential...
Here, we describe two-cell homologous recombination (2C-HR)-CRISPR, a highly efficient gene-editing method based on introducing CRISPR reagents into embryos at the two-cell stage, which takes advantage of the open chromatin structure and the likely increase in homologous-recombination efficiency ...
Northern blot analysis also confirmed the expression of the PAF receptor in the CFW mouse preimplantation two-cell-stage embryo. PAF treatment of the two-cell-stage CFW mouse embryo resulted in a fourfold increase in intracellular calcium over background levels. 展开 ...
Indeed, although the zygote and two-cell stage in mouse embryos last ∼18–20 h each, subsequent cell cycles are only ∼10–12 h (ref. 65), and because PEmbryo exhibits low error frequencies, each new cell cycle should provide new, editable templates, which could account for high ...
Indeed, although the zygote and two-cell stage in mouse embryos last ∼18–20 h each, subsequent cell cycles are only ∼10–12 h (ref. 65), and because PEmbryo exhibits low error frequencies, each new cell cycle should provide new, editable templates, which could account for high ...
L. First.Culture of porcine embryos from the one- and two-cell stage to the blastocyst stage in sheep oviducts.Theriogenology. 1991Prather, R.S., Sims, M.M. and First, N.L. Culture of porcine embryos from the one- and two-cell stage to the blastocyst stage in sheep oviducts. ...
Results: Here we report a dynamic and transcription-independent pattern of WNT signaling in the two-cell stage of H. robusta. Surprisingly, HRO-WNT-A is first expressed in a stochastic manner, such that either AB or CD secretes the protein in each embryo. This stochastic phase is followed ...
We found that the Trps1 mRNA level peaked at the one-cell stage while TRPS1 protein did so at the two/four-cell stage. Knockdown of Trps1 by the microinjection of Trps1 siRNA reduced the developmental rate of mouse preimplantation embryos by approximately 30%, and increased the expression ...
maternal plag1 deficiency delays zygotic genome activation and two-cell stage embryo development 2 running title plag1 controls genome activation 3 来自 dx.doi.org 喜欢 0 阅读量: 20 作者: E Madissoon,§,A Damdimopoulos,S Katayama,K Krjutskov,E Einarsdottir,K Mamia,BD Groef,O Hovatta,...
(Table 3), whereas when a small amount of supernatant (approximately 10 pl) was injected into MII oocyte and activated, although most of them showed normal pronuclear formation, the development of the 2-cell stage embryo remarkably decreased, and the rate of blastocyst formation was only 2%...