The finally engineered short sequence consists of only 8 amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) and was named Strep-tag? II. To optimize binding properties, also streptavidin has been engineered to obtain StrepTactin?. Thus, the optimal binding partners have been found: The (Twin-)...
This approach uses short, homologous DNA sequences that flank the tagging cassette to direct recombination. Here, we constructed a set of plasmids, whose sequences were optimized for codon usage in yeast, for Strep-tag II and Twin-Strep tagging in S. cerevisiae. Some plasmids also contain ...