Categories included under aggregate turnover to computer GST in India are as follows: As per section 2 (6) of the MGL, aggregate turnover includes the aggregate value of: (i) all taxable and non-taxable supplies (ii) exempt supplies, and (iii) exports of goods and/or service of a pers...
A defining characteristic of young ventures is that they are more likely to experience periods of change (of both a positive and negative nature) than are established organizations. This could result in a misalignment between employees’ expectations when hired and actual work experiences. Based on ...
we proposed that ATG14 may act as a receptor on LDs. Using in vitro GST pull-down assays and in vivo co-IP assays, we confirmed that ATG14 binds with LC3A, LC3C, GABARAP, and GABARAPL2, but not LC3B or GABARAPL1 (Fig.2a
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(C) Sequence mapping of proteins used in in vitro kinase assays. Sequence coverage of Atg proteins purified from GST- and TAP-tagged yeast strains is shown. Trypsin was used as protease for bottom-up proteomics experiments. Error bars indicate standard deviations (n = 3). (D) Workflow of ...
g–i Pulldown from lysates of HEK293T cells co-expressing eGFP-MTM1, -MTMR1, or -MTMR2, and FLAG-MTMR13 or FLAG using immobilized active GST-Rab35•GTP. g Representative immunoblot as in d. Input: 5% of total protein. h, i Quantification of representative data in g. Bound protein...
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None of these species showed evidence of the LG19 system found in other species of is significant, and Clade 1. Tropheus duboisi Dunn's tests identify LG9 ahsasdnisotinobctv.iTouhserseiganrael4intothpe1F%STwpilnodt.oTwhsetKhaWt stuegstgefostr heterogeneity an XY system on LG9, but the ...
Plasmids and Transfections—BimEL, BimL, BimS, and fragments of BimEL were expressed as GST fusion proteins in pGEX-4T1 or as HA-tagged proteins in pCAN-HA (a derivative of pCDNA3 that includes an ATG and in-frame HA tag at the 5′ end of the MCS). GST-Bim constructs were expressed...
(B) Western blots of whole-cell lysates (left) or IP (right) from cells co-expressing FLAG-STIM1 CC1-CAD, FLAG-STIM1 CT, FLAG-STIM1 CAD, or FLAG-STIM1 CC1 and GFP-Orai1 in HEK293T WT and P1 KO cells. (C) In vitro binding of recombinant PGRMC1 to recombinant GST-STIM1 CC1...