Human TorsinA (residues 51–332), N-terminally fused with a human rhinovirus 3C protease cleavable MBP tag, was cloned into a modified ampicillin resistant pETDuet-1 vector (EMD Millipore). Mutations were introduced by site-directed mutagenesis. The E. coli strain LOBSTR(DE3) RIL (Kerafast)48...