For electron cryomicroscopy, 3 μl of ~0.06 mg/ml TorsinA polymers were applied to glow-discharged Quantifoil R1.2/1.3 400 mesh Cu holey carbon grids (Quantifoil, Germany), blotted (8 s) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Da...