is that the proteins are enriched for Intrinsically Disordered Regions (IDRs)5. IDRs are protein regions characterized by an inability to adopt a stable tertiary structure; instead, IDRs exist in a collection of interconverting conformations6. IDRs participate in diverse regulatory pathways, are freque...
1a). Finally, p-ERK1/2 staining in the small intestine was abundant in the transit-amplifying zone of the crypt, as recently reported15 (Supplementary Fig. 1d). This specific staining pattern was only observed in fl/fl mice, whereas p-ERK1/2 immunoreactivity in ΔIEC mice was only ...
In the ELISA-based assay, biotinylated RNaseB was incubated with YPng1 in the presence of potential inhibitors. The mixture was transferred to streptavidin-coated multiwell plates where the glycosylation status of RNaseB was probed with fluorophore-conjugated ConA. When YPng1 is inhibited, greater...
Cellulase activity in diplogastrid nematodes. The cellulase activity assays were performed on carboxymethylcellulose agar plates [3, 23]. Clear halos were detected in the supernatants of P. uniformis, P. entomophagus, P. aerivorus, P. pseudaerivorus, P. sp. 11, P. sp. 15, and K. sud...
Accumulation of the tau protein in fibrillar intracellular aggregates is a defining feature of multiple neurodegenerative diseases collectively referred to
SP7/Osterix is a transcription factor critical for osteoblast maturation and bone formation. Homozygous loss-of-function mutations in SP7 cause osteogenesis imperfecta type XII, but neomorphic (gain-of-new-function) mutations of SP7 have not been reporte
Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron degenerative disease. ALS-affected motor neurons exhibit aberrant localization of a nuclear RNA binding protein, TDP-43, into cytoplasmic aggregates, which contributes to pathology via unclear m
Cells were incubated for 15 min in PBS containing 50 µM sensor 1, then washed 3 times using warm PBS saline. Plates were exposured under a UV lamp for 10 min to activate 1 prior to imaging. An Olympus FluroView V10i confocal microscope was used for all the imaging. For the ...
Gain-of-function p53 mutants such as p53-R175H form stable aggregates that accumulate in cells and play important roles in cancer progression. Selective degradation of gain-of-function p53 mutants has emerged as a highly attractive therapeutic strategy t
HeLa and RPE CRISPRi/a cells were seeded in 96-well plates and cultured for 0, 24, 48, 72 and 96 h (HeLa: 1500 cells; RPE: 2500 cells). Cell viability was measured at indicated time points. For motility assays, cells were seeded into 24-well plates with a low confluence and were...