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faster innaa10Δcompared to the WT. Next, we compared the total distribution of the normalized turnover rates of proteins with the corresponding Nt-peptides determined in WT (Fig.3C) andnaa10Δstrains (Fig.3D). Interestingly, we found no statistical difference between the normalized turnover r...
To quantify the defective mitotic centrosomes in fixed cells, randomly selected mitoses were analyzed, following depletion of ULK1, ATG7 or ATG5, included as an additional control for autophagy specificity (see Supplementary Fig.1 A). While control cells showed a low baseline of abnormalities, de...
plants Article AtXRN4 Affects the Turnover of Chosen miRNA*s in Arabidopsis Yan Liu 1,2,†, Wenrui Gao 1,3,†, Shuangyang Wu 1,2,† , Lu Lu 1, Yaqiu Chen 1,4, Junliang Guo 1,5, Shuzhen Men 3 and Xiaoming Zhang 1,2,* 1 State Key Laboratory of Integrated Management of ...
Introduction Reactive oxygen species (ROS) [1] exert important physiological roles, being involved in processes such as cell signaling and immune responses [2,3], but on the other hand, their buildup in cells is linked to the mechanisms leading to a number of pathological conditions including ...
2.3.6. Selectivity of Sensor 1 In a black 96-well plate, sensor 1 (50 µM) was separately mixed with solutions (1 mM) of various competing substrates (GSSG/Cys/Glu/Asp/2-mercaptaethanol/Ca2+/Zn2+/Na+/K+) in 0.1% DMSO in H2O. The mixtures were incubated in the dark for 30 ...