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faster innaa10Δcompared to the WT. Next, we compared the total distribution of the normalized turnover rates of proteins with the corresponding Nt-peptides determined in WT (Fig.3C) andnaa10Δstrains (Fig.3D). Interestingly, we found no statistical difference between the normalized turnover r...
To quantify the defective mitotic centrosomes in fixed cells, randomly selected mitoses were analyzed, following depletion of ULK1, ATG7 or ATG5, included as an additional control for autophagy specificity (see Supplementary Fig.1 A). While control cells showed a low baseline of abnormalities, de...
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A better understanding of proteostasis in health and disease requires robust methods to determine protein half-lives. Here we improve the precision and accuracy of peptide ion intensity-based quantification, enabling more accurate protein turnover determ
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