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MDCK cells do not move once they reach confluence, but they do move in response to wounding (35). Cells might need to disassemble their stress fibers to allow them to move and repair the wound. We examined the fate of actin stress fibers following wounding of epithelial sheets in WT ...
iBACs were seeded into 6-well plates 12 h before transduction using 3 × 104 cells/well (around 30% confluence). Cells were infected for 16 h with a multiplicity of infection of 10 in complete medium containing 8 μg/ml Polybrene (Sigma). After transduction, the infection medium was ...
These cells were plated and grown to confluence in Dulbecco's modified Eagle's medium supplemented with 10% calf serum as described previously (4). Four days postconfluence, the medium was replaced with Dulbecco's modified Eagle's medium supplemented with 0.5% essentially fatty acid-free bovine ...