Single cell, RNA-seq, Tissue dissociation, Gene expression, Quality control, Breast cancer, Ovarian cancer, Tumor microenvironmentBackgroundSingle-cell RNA sequencing (scRNA-seq) is a powerful tool for studying complex biological systems, such as tumor heterogeneity and tissue microenvironments. However...
Tumor Dissociation Kit, human,130-095-929,MiltenyiBiotec: overview: The Tumor Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from human tumor tissue. The kit is optimized for high yield of tumor cells and tumor-infiltrating lymphocytes, while pres...
这里的标准是:1)single-cell 测序数据要和bulk-seq数据的相关性高(相关性分析);2)测序深度,测序数据量要够(过滤后查看genes和UMI数量);3)为避免批次效应,作者使用基于熵的测量方法来测定:细胞最近邻在不同样本中的分布有多均匀(To quantify sample-to-sample variation and the extent of possible batch effects...
借助单细胞多组学可以深入分析各种应用,包括免疫学、肿瘤学和新陈代谢。BD Biosciences提供了各种单细胞多组学工具,如BD Rhapsody™ Single-Cell Analysis System和BD®AbSeq Assays,将RNA和蛋白质生物标记物表达和强大的生物信息学工具结合在一起,如FlowJo™ v10 Software和SeqGeq™ v1.6 Software,可以帮您获取...
Tissue dissociation and single-cell sample preparation Submandibular glands and primary tumor samples were collected, minced and dissociated with a GentleMacs Dissociator (Miltenyi Biotec) in digestion buffer (DMEM/F12 1:1 (Invitrogen), 1.67 mg/ml collagenase (Invitrogen), 1.33 mg/ml hyaluronidas...
Tissue dissociation and single-cell suspension preparation Fresh samples were stored in the GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies, Nanjing, China) at 2–8 °C immediately after being collected by needle biopsy or bronchoscopy. Prior to dissociation, tissue samples were washed...
2. Tissue dissociation and single-cell sample preparation 3. Drop-seq procedure, single-cell and bulk library generation, and sequencing 4. CITE-seq experiments 5. Immunostainings 6. Processing and analysis of single-cell RNA-seq data ,生信工程师上场,原文写得非常非常详细。 框架: (1) ...
After dissociation, single-cell suspensions were filtered using a 40-μm cell strainer to remove large pieces of debris. Red blood cells (RBC) were removed by incubating the cells with RBC lysis buffer (ThermoFisher Scientific, Carlsbad, CA). Cells were then counted and evaluated for viability...
TheTumorDissociationKithasbeendevelopedforthegentleandrapidgenerationofsingle-cellsUSPensionsfromhumantumortissue.Thekitisoptimizedforhighyieldoftumorcellsandtumor-infiltratinglymphocytes,whilepreservingimportantcellsurfaceepitopes.Thekitisideallysuitedforthetime-savingandreproducIBLepreparationofsingle-cellsuspensionsincombinat...
The tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) is a complex ecosystem that drives tumor progression; however, in-depth single cell characterization of the PDAC TME and its role in response to therapy is lacking. Here, we perf