The dissociation chamber design was motivated by the goal of disrupting tissue while maintaining structural integrity of cells. Tumor models of increasing complexity were used to characterize the device. Concentration limits, ideal flow rates, cell viability, and biomarker integrity were evaluated. Tests...
Fig. 1: Single-cell RNA sequencing of lung adenocarcinomas. A Schematic representation of the workflow, ten normal (blue) and ten tumor (red) tissue samples were obtained from 12 patients. B, C UMAPs based on the top 15 principal components of all single-cell transcriptomes after filtering,...
Tissue dissociation and single-cell sample preparation Submandibular glands and primary tumor samples were collected, minced and dissociated with a GentleMacs Dissociator (Miltenyi Biotec) in digestion buffer (DMEM/F12 1:1 (Invitrogen), 1.67 mg/ml collagenase (Invitrogen), 1.33 mg/ml hyaluronidas...
Tumor Dissociation Kit, human,130-095-929,MiltenyiBiotec: overview: The Tumor Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from human tumor tissue. The kit is optimized for high yield of tumor cells and tumor-infiltrating lymphocytes, while pres...
The Tumor Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from human tumor tissue. The kit is optimized for high yield of tumor cells and tumor-infiltrating lymphocytes, while preserving important cell surface epitopes. The kit is ideally suited for...
美天旎Tumor Dissociation Kit Tumor Dissociation Kit, human (130-095-929) 货号 130-095-929 描述 The Tumor Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from human tumor tissue. The kit is optimized for high yield of tumor cells and tumor-in...
sequencing. However, the integration of several datasets has the advantage of mitigating biases related to cell preparation and dissociation, such as the preferential liberation of specific cell types during tissue dissociation. Additionally, our ligand–receptor analysis relies on a limited database of ...
TheTumorDissociationKithasbeendevelopedforthegentleandrapidgenerationofsingle-cellsUSPensionsfromhumantumortissue.Thekitisoptimizedforhighyieldoftumorcellsandtumor-infiltratinglymphocytes,whilepreservingimportantcellsurfaceepitopes.Thekitisideallysuitedforthetime-savingandreproducIBLepreparationofsingle-cellsuspensionsincombinat...
To compare cell type frequencies between tumor and non-tumor tissue, we applied the PhenoGraph algorithm (Levine et al., 2015), which partitioned our high-dimensional single-cell data into 42 clusters (Figures S1M and S1N; STAR Methods). Marker expression profiles reliably assigned these ...
Cancer-associated fibroblasts (CAFs) are a heterogeneous cell population that plays a crucial role in remodeling the tumor microenvironment (TME). Here, through the integrated analysis of spatial and single-cell transcriptomics data across six common can