Pathology DL: CNNs Tumor proportion score of PD-L1 expression NSCLC Accuracy= 0.9326; specificity= 0.9641 ICB 66 Genomics DL: CNNs Immune properties of genes Gastric cancer Accuracy= 0.68 N/A 53 Pathology DL: High-Resolution Network version 2 (HRNetV2) Computer-assisted PD-L1 quantitation NSCLC...
TIL scoring was performed using the Salgado scoring criteria and PDL-1 IHC staining was assessed based on Tumor proportion score (TPS) and Combined Proportion score (CPS). Additional slides were stained with antibodies for CD3, CD8, CD56, CD19, FOXP3, cytokeratin, and DAPI. Whole slides were...
To further gain insight into the underlying anti-tumor mechanism of KEAP1, we performed mass spectrometry (MS) based protein interaction analysis in HEK293T cells to determine the KEAP1 interacting proteins. The MS results highlighted PD-L1 (CD274) as one of the robustly interacting proteins with...
The proportion of PD-L1 positivity was 14.5% and 93.2%, respectively (Fig. 5A and B). Fig. 5 Representative flow cytometry analysis of the expression of PD-L1 and PD-1. PD-L1 expression on A MCF-7 and B MDA-MB-231 cells. C PD-1 expression on PBMCs after ConA stimulation for 6...
(Supplementary Fig.8). However, although the proportion of PD-L1-positive exosomes from each patient varied between 10 and 70% (Supplementary Fig.9), this proportion was correlated with the PD-L1 expression level in the cancer tissues from the same patients (Fig.5a, b). Next, we examined...
Trp53R172H/+ mouse pancreatic ductal epithelial tumor promotes neutrophil recruitment and down-regulates CD3+ T cells, CD8+ T cells, and CD4+ T helper 1 cells to resistant immune therapy [48]. And our results showed that the immunescore in patients with wild-type p53 appeared higher compared...
After 5 days, when the tumor volume was approxi- mately 4–5 mm3, the mice were randomly divided into six groups, with half of the ShCon and ShTMPRSS2 mice treated with 150 U/L PD1/PDL1 inhibitor BMS-1 (con- centration 500 mg/mL; i.p.) (MCE Cat. No. HY-19991) every...
Another hypothesis is that the S.score predicts the proportion of sarcomatoid-like cells, which may not have completely a characteristic sarcomatoid cell morphology. Gene expression-based signatures to predict physiological process or prognosis such as the E/S.score or the CV score (CLDN15/VIM) ...
(D) GSEA plots for oxidative stress-related gene sets in Pdl1.WT vs. Pdl1−/− DC2.4 cells. NES, normalized enrichment score; FDR, false discovery rate. (E) Representative transmission electron microscopy (TEM) images showing the morphology of BMDCs after doxorubicin treatment. Scale bar,...
Although PD-L1 expression levels are employed to determine the tumor proportion score and assess patient response to programmed cell death protein 1 (PD-1)/PD-L1 inhibition [9]. Both upregulated and downregulated PD-L1 expression had been reportedly associated with favorable PD-(L)1 therapeutic ...