tumor cell‐conditioned mediumStem cell-conditioned medium (CM), which contains angiogenic factors that are secreted by stem cells, represents a potential therapy for ischemic diseases. Along with stem cells, tumor cells also secrete various angiogenic factors. Here, tumor cells as a cell source of...
An ELISA for human EGF was performed with MB231-LEC, MB231-HUVEC and normal LEC conditioned media (Fig. 2a). hEGF was highly expressed in MB231-LEC compared to other groups (Fig. 2a). We estimated that 1,000 MB231-LEC cells expressed 1.1 ± 0.16 pg hEGF. MB231 cell proliferation...
(B). Splenocytes isolated on the indicated day after 4T1 cell inoculation were cultured with the conditioned medium of 4T1 cells and IFN-γ and TNF-α in the culture medium were measured. The data are expressed as the percentage of the findings without the conditioned medium. The spleno...
Previously, the unusual anti-tumor role of TAM at the interface of cancer cell-derived apoptotic cells was mentioned [95]. It is known that phagocytes maintain tissue homeostasis by clearance of apoptotic cells. Such an effect action has also been described in conditioned medium from macrophages ...
A cell motility-stimulating factor has been isolated, purified, and partially characterized from the serum-free conditioned medium of human A2058 melanoma cells. We term this activity "autocrine motility factor" (AMF). AMF has the properties of a protein with an estimated size of 55 kDa. At ...
To reveal the molecular mechanism by which Jagged1-expressing tumors recruit macrophages, we utilized an in vitro cell migration assay. WT;PyMT and Jagged1;PyMT mice were sacrificed to collect tumor tissue, which was cultured in vitro. Conditioned medium (CM) from primary tumor cell culture was...
The DC2.4 mouse dendritic cell line was purchased from Merck Millipore (Darmstadt, Germany), and the Jurkat/NFAT-Luc cell line was purchased from InvivoGen (Hong Kong, China). The mouse pancreatic cancer cell lines were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning Inc., ...
Synergism of leukemic blast growth factors in medium conditioned by human bladder carcinoma cell line 5637 Int J Cell Cloning, 5 (1987), p. 504 CrossrefView in ScopusGoogle Scholar 52 Kruys V, Marinx O, Shaw G, Deschamps J, Huez G Translational blockade imposed by cytokine derived UA-rich...
Results The expression levels of TGF-β1, Gli2 and IGF-II and the cell proliferation, migration and invasion abilities were elevated in Huh-7 cells treated with TAMs-CM. TGF-β1 was upregulated in the conditioned medium and was found to be involved in the promotion of migration, invasion...
(cross)-presentation to naïve T cells. Interferon-α-conditioned DCs (IFN-DCs) exhibit marked phagocytic activity and the special ability of inducing Ag-specific T-cell response. Here, we have developed a novel microfluidic platform recreating tightly interconnected cancer and immune systems with ...