The first large-scale study demonstrating how phenotypic testing can be replaced by WGS for first-line drug testing. Google Scholar Gardy, J. L. et al. Whole-genome sequencing and social-network analysis of a tuberculosis outbreak. N. Engl. J. Med. 364, 730–739 (2011). CAS PubMed ...
A recent theory has postulated a continuum spectrum of tuberculous pleurisy, suggesting that TB empyema are predominant in lymphocytes during initial testing. However, the fluid becomes predominantly neutrophilic as the disease progresses, along with the development of loculations and positive spill cultur...
However, in South Africa, the impact of the 2020 national COVID-19 lockdown on HIV testing and treatment in KwaZulu-Natal, where 1•7 million people are living with HIV, showed that ART treatment provision was generally maintained during the lockdown, but HIV testing and ART initiations ...
This is followed by further Mtb specific tests or drug susceptibility testing (i.e. nucleic acid amplification tests such as the Gene Xpert MTB/RIF [4]). Diagnosis of TB in most low- and middle-income countries continues to rely on sputum smear microscopy for acid-fast bacilli (Ziel-...
However, such testing has not been routinely required in adjacent counties in the AF zone where bTB is either endemic, or at least periodically present, in wild deer at very low prevalence. Slaughter surveillance, while more cost effective, may not detect infected herds until several years after...
Moreover, phenotypic drug-susceptibility testing to first- and second-line anti-TB drugs was done using the proportion method57. The strain isolated in Uganda was not available for phenotypic characterisation. Targeted- and whole-genome sequencing of the Rwandan strain. Targeted sequencing was ...
High cost More laboratory resources required Complicated process of lymphocyte separation Lack of prospective studies Screening test accuracy Neither tuberculin skin testing nor IGRA testing is sufficiently sensitive to rule out TB infection.[69] Approximately 20% of patients with active TB, particularly ...
Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev. 2006;19(1):165–256. 19. Palomino JC. Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur Respir J. 2005;26(2): 339–50. 20...
The shorter all-oral BDQ-containing regimen is recommended in patients with MDR-TB (with at least confirmed RIF resistance) who fulfill the following eligibility criteria: (1) resistance to fluoroquinolones has been excluded as fluoroquinolones susceptibility testing needs to be undertaken before the ...
This is followed by further Mtb specific tests or drug susceptibility testing (i.e. nucleic acid amplification tests such as the Gene Xpert MTB/RIF [4]). Diagnosis of TB in most low- and middle-income countries continues to rely on sputum smear microscopy for acid-fast bacilli (Ziel-...