New England Research Institutes, Watertown, MA, USA;;Director, Sexual Medicine, Alvarado Hospital, San Diego, CA, USA;;Bayer Corporation, West Haven, CT, USA;Xing-Yue HuangNew England Research Institutes, Watertown, MA, USA;;Director, Sexual Medicine, Alvarado Hospital, San Diego, CA, USA;;...
Initially the cDNA was synthesized using oligo(dT)20 primer with 1.0 μg of total RNA in a final reaction volume of 20 μl using the Superscript™ III First-strand synthesis system for reverse transcriptase-polymerase chain reaction (RT-PCR) (Invitrogen Corp., San Diego, CA, USA). Using...
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The concentration of the DNA recovered from the agarose gel (see above) were quantified with a Qubit Fluorometer, diluted appropriately, and loaded onto a HiSeq 2500 machine (Illumina, San Diego, USA). Between 6 and 14 million reads were obtained for each RNA sample, and on average about ...
E. TyszkaJ.A. RamosICALEO '99, vol. 87, part 1: Laser materials processing conference, November 15-18, 1999, San Diego, CA, USA