1. Login or Register with R&D Systems to submit a review 2. Fill out the product review form 3. You will be emailed your reward within 2 weeks Terms and Conditions: These terms and conditions contain important information regarding the Product Review Program and, by participating in such Progr...
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB13981 ). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human TSLP DuoSet ELISA Kit (Catalog # DY1398...
This ELISA-based chemiluminescent assay provides robust signal-to-noise ratios and is provided in a convenient kit format. Quality testing ensures lot-to-lot consistency in performance. Figure 5. Dose-dependent inhibition of TSLP:TSLPR binding by Anti-TSLP Neutralizing Antibody (#102138). View ...
Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999) Stop Solution:2 N H2SO4(Catalog #DY994) Microplates: R&D Systems (Catalog #DY990) Plate Sealers:ELISA Plate Sealers (Catalog #DY992) ...
Antibodies in B cell culture supernatant was quanti- fied by IgG Elisa kit (CUSABIO), IgG4 Elisa kit (CUSABIO) and IgE Elisa kit (Bethyl Laboratories). Histology, immunohistochemistry and immunofluorescence Formalin-fixed, paraffin-embedded samples were cut into consecutive 3-μm thick sections....
TSLP concentrations were measured using a human TLSP ELISA kit (R&D Systems, Minneapolis, USA) according to the manufacturer’s instructions. The standards and an internal control were conducted in triplicate. The intra-assay coefficient of variation (CV) was <13.5 % and inter-assay CV was 22...
The concentrations of alarmins (IL-25, IL-33 and TSLP) and IL-23 contained in the sputum supernatant were assessed by ELISA multiplex using Fluorokine® Multianalyte Profiling (MAP) Kits and a Duoset kit was used for IL-36 (R and D systems, Minneapolis, USA) according to the manufactur...
2.7. ELISA Quantification of mouse TSLP concentration in serum, total protein from skin biopsies, and media from ear explant cultures was performed by enzyme-linked immunosorbent assay (ELISA) according to the DuoSet ELISA Development kit protocol or Quantikine ELISA Development kit protocol (R&D Syste...
Institutional Review Board Statement Not applicable. Informed Consent Statement Not applicable. Data Availability Statement Data available upon reasonable request. Conflicts of Interest The authors have no conflict of interest to declare. References
ELISA Immunoreactive TSLP and CCL17 within the supernatants were quantified using DuoSet TSLP ELISA assays (R&D Systems) and TARC/CCL17 ELISA assay kit (Abcam), according to the manufacturer’s instructions. DNA construction To induce constitutive HIF-1α expression regardless of oxygen tension, the...