Data are mean ± s.d., n = 18 biologically independent samples, two-tailed unpaired Student’s t-test. b, From same experiment, PKG1 activation is found to be independent of the form of TSC2 expressed; n = 6 biologically independent samples, box and whisker and raw data plots. Data ...
Although mammalian target of rapamycin 1 (mTORC1) negatively regulates autophagy in cultured cells, how autophagy impacts mTORC1 signalling, in particular in an in vivo setting, is less clear. Here we show that autophagy supports mTORC1 hyperactivation i