Protocol for TSA Vivid Fluorophore Kits Introduction TSA Vivid reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology ap
Multiplex TSA-plusFluorescence IHC Combined with Spectral Image Analysis for PI3K Pathway Dysregulation in Prostate Cancerdoi:10.1038/modpathol.2015.16Ying Huang2015 United States & Canadian Academy of Pathology Annual Meeting
对于许多免疫组织化学(IHC)应用,传统的酶促扩增程序足以实现足够的抗原检测。但是,有几个因素限制了这些程序的敏感性和实用性。酪酰胺信号放大(TSA)已被证明是一种特别通用且功能强大的酶放大技术,具有更高的测定灵敏度。TSA基于HRP在低浓度过氧化氢存在下将标记的含酪胺底物转化为可与HRP或附近的酪氨酸残基共价结合...