方法 TSA(300 nm/L)处理人肝癌SMMC-7721细胞,MTT法,TUNEL染色法分别检测细胞生长抑制及凋亡情况,甲基化特异性的PCR(methylation-specificPCR,MSP)检测处理前后E-cad启动子区CpG岛甲基化状态;Western blot检测处理前后E-cad及DNMT3b表达水平的变化.结果 TSA能抑制人肝癌SMMC-7721细胞生长并诱导其发生凋亡,与对照组...
2.2 胃癌细胞系经5-Aza-dC和TSA处理前后的p16、hMLH-1基因启动子甲基化分析 以研究对象基因组DNA模板, 经MSP扩增后, 无甲基化条带而出现非甲基化条带, 判定为非甲基化; 无非甲基化条带而出现甲基化条带, 判定为甲基化; 甲基化条带和非甲基化条带同时出现, 判定为半甲基化(图2). (1)在MGC-803和MKN-...
The special security lane for passengers with an MSP Reserve reservation, photo courtesy of MSP Today, it’savailable at nine U.S. airports– though not at every terminal. It’s also available at a handful of Canadian airports as well as a few in Europe. Denver (DEN) Houston-Intercontinent...
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TSA安保考察是美国国土安全部(DHS)下辖单位美国运输安保局以美国发《外国承运人模式安保方案》(MSP)和《紧急修正案》(EA)为标准,对外国航空公司飞美航班安保工作开展的高标准检查。对外国航空公司而言,要想运营飞美航线,通过TSA检查是必不可少的要求。然而,如此高难度、高标准的考察迎检工作,留给深航准备的时间仅有...
There are some tips and tricks for getting through the TSA line as quickly as possible when travelling.
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cific-polymerasechainreaction(MSP),andthe expressionlevelsofp16andhMLH-1genewere detectedusingreversetranscription-polymerase chainreaction(RT-PCR). RESULTS:Reexpressionorraisedexpressions ofp16andhMLH-1mRNAwasdetectedin MGC-803andMKN-45gastriccancercelllines ...