The titration of the – SH groups of beef pancreas tryptophanyl-tRNA synthetase by N- ethylmaleimide and the consequent effect on the exchange and transfer reactions of the enzyme were examined. The 16 sulfhydryl groups of the native dimeric enzyme do not react homogeneously at pH 7, with 1 ...
1. 1.|Previous investigations have shown that 伪-lactalbumin undergoes a structural change at acid pH which involves molecular swelling, alteration of the ... MJ Kronman,LG Holmes,FM Robbins - BBA - Protein Structure 被引量: 275发表: 1967年 Reactivities of the tryptophan residues of alpha-lac...
(HPBCD) in water, pH 7.2) or vehicle (40% HPBCD in water, pH 7.2, bid) after magnetic resonance imaging (MRI)-based randomization of tumor volume at d8 after intracranial injection of GL261 IDH-WT or GL261 IDH1-R132H tumors (n = 19 IDH-WT tumor-bearing mice versus n = ...
Modification of tryptophan residues in castor bean hemagglutinin (CBH) with N-bromosuccinimide (NBS) was investigated in detail. Tryptophan residues accessible to NBS increased with lowering pH and six tryptophan residues/mol were oxidized at pH 3.0, while two tryptophan residues/mol were oxidized at...
People can use the following data to convert to the molecule structure. 1. SMILES:O=C(O)[C@H](N)Cc2c1ccccc1nc2 2.?InChI:InChI=1/C11H12N2O2/c12-9(11(14)15)5-7-6-13-10-4-2-1-3-8(7)10/h1-4,6,9,13H,5,12H2,(H,14,15)/t9-/m1/s1 The following are the toxicity ...
When native protein was loaded, IasA eluted as a single peak at 14.4 mL, corresponding to the size of a homodimer (Additional file 1: Figure S14). This result is consistent with previously described homodimeric AAADs and AASs [30]. When the in silico modeled structure of P. mexicana ...
Fig. 4. Crossed eye stereo structure of the aminoacrylate complex of tryptophan synthase from Salmonella typhimurium, from 6VNT.pdb. The red spheres are waters. Hydrogen bonds are indicated with blue dashes. The X-ray diffraction data give an electron density map, but do not give us the posi...
(Fig.3aand Supplementary Table4). Consistent with the NetSurfP predictions, the structure is composed primarily of α-helices that are arranged into an extended three-helical bundle with small helical domains at each end. The conserved tryptophan residues that define the domain are distributed ...
Upon BL illumination, LOV2-Jα unfolds, perturbing the structure of the effector protein, which leads to its inactivation. Using a similar principle, proteins can also be activated upon light illumination if the light sensor is inserted within the autoinhibitory domain of the protein; perturbation...
Thus, the equilibrium model developed here for describing the formation of structure during folding faithfully captures snapshots of transient kinetic intermediates observed on the folding pathway of barstar. A comparison of the refolding kinetics at pH 7, when refolding is initiated from the D, P,...