(1987) Mechanism of tryptophan indole-lyase: insights from pre-steady-state kinetics and substrate and solvent isotope effects. J. Am. Chem. Soc. 111, 727-730.Phillips RS (1989) The mechanism of tryptophan indole-lyase: insights from pre-steady-state kinetics and substrate and solvent isotope...
Wikipedia tryp·to·phan syn·thase a nonmammalian hydro-lyase condensingl-serine indole-3-glycerol phosphate to producel-tryptophan and glyceraldehyde phosphate; pyridoxal phosphate is required; it will also reactl-serine with indole. Synonym(s):tryptophan desmolase,tryptophan synthetase ...
G. Proton transfer and carbon–carbon bond cleavage in the elimination of indole catalyzed by Escherichia coli tryptophan indole-lyase. J. Am. Chem. Soc. 122, 1008–1014 (2000). CAS Google Scholar Seyedsayamdost, M. R., Reece, S. Y., Nocera, D. G. & Stubbe, J. Mono-, di-, ...
Tryptophan synthase is a multienzyme α2β2 complex that catalyzes the retro-aldol cleavage of indole-3-glycerol phosphate (IGP) to indole and d-glyceraldehyde-3-phosphate (Eq. 4) in the α-subunit and the irreversible condensation of indole and l-serine in the β-subunit (Eq. 5) (Miles...
Target protein abundance was measured by PRM throughout the experiment (Additional file6). Tryptophan biosynthesis pathway protein levels, PHOSPHORIBOSYLANTHRANILATE TRANSFERASE 1 (PAT1), INDOLE-3-GLYCEROL PHOSPHATE SYNTHASE (IGPS), TRYPTOPHAN SYNTHASE ALPHA CHAIN (TSA1), and TRYPTOPHAN SYNTHASE BETA-SUB...
Examines the effects of alpha-deuteration and of aza and thia analogs of L-tryptophan on the formation of intermediates in the reaction of Escherichia coli tryptopha indole-lyase. Performance of single-wavelength stopped-flow kinetic measurements; Effects of benzimidazole on the pre-steady-state reac...
The interaction of the mutant tryptophan indole-lyase (TIL) from Proteus vulgaris Y72F with the transition state analogue, oxindolyl-l-alanine (OIA), with ... NG Faleev,LN Zakomirdina,MM Vorob'Ev,... - 《Biochimica Et Biophysica Acta Bba International Journal of Biochemistry Biophysics & ...
Germinating seedlings of Catharanthus roseus produce monoterpenoid indole alkaloids as a result of a transient increase of tryptophan decarboxylase (TDC) activity. The influence of auxins on this transient rise of TDC activity was studied. External application of indolebutyric acid or 2,4-dichlorophenox...
In hTDO, the conserved H76 residue is believed to act as an active site base to deprotonate the indole NH group of Trp, the initial step of the Trp oxidation reaction. In hIDO, this histidine is replaced by a serine. To investigate the role of the H76, we have studied the H76S ...
Explore all metrics Abstract A plant-specific biogenic amine, serotonin, was produced by heterologous expression of two key biosynthetic genes, tryptophan decarboxylase (TDC) and tryptamine 5-hydroxylase (T5H), inEscherichia coli. The native T5H, a cytochrome P450 enzyme, was unable to be functiona...