Tryptone Soya Yeast Extract Agar is recommended for conformation of Listeria in Henry`s light (1). It is also recommended by ISO Committee under the specification ISO 10560:1993.Composition:Ingredients Grams/Litre Casein enzymic hydrolysate 17.0 Papaic digest of soyabean meal 3.0 Sodium chlorid...
Tryptone Soya Broth is often for the tube dilution method of antibiotic susceptibility testing. The addition of a small amount of agar ( approx. 0.05-0.2% 05040, add before sterilisation) renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium species. The ...
Blend 25 grams food sample to be tested in 224 ml Modified Soyabean Bile Broth and incubate with shaking (about 100 rpm) at 37°C for 18-24 hours. Prepare dilution of the enrichment culture with phosphate buffer and spread 0.1 ml of each dilution on HC Agar plates and incubate at 43...