胰酶消化液(Trypsin Solution)产品及特点: 胰蛋白酶(trypsin,胰酶)使精氨酸或赖氨酸的羧基和其他氨基酸的氨基之间的多肽链发生水解,导致细胞间质水解而使细胞或组织块消化为分散的单个细胞。而乙二胺四乙酸二钠(EDTA)通过与二价钙、镁离子结合,也能使细胞分散。所以胰酶(含EDTA或不含EDTA)的消化液常用于细胞生物学...
Trypsin retains most of its activity in 2.0 M urea,2.0 M guanidine HCl, or 0.1% (w/v) SDS.8 Trypsin is reversibly denatured at high pH (above 11), byprecipitation with TCA, or by high concentrations ofurea (greater than 6.5 M).3 In order to abolish alltrypsin activity, heating at ...
The presence of Ca2+(20 mM) will also retard trypsin's ability to selfdigest itself (autolysis) and will maintain the stability of the trypsin in solution.2,9 Trypsin retains most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.13 Trypsin is reversibly ...
Trypsin/EDTA Solution (TE) Certificates SDS Gibco™ Trypsin/EDTA Solution (TE) Gibco™ Trypsin solutions are made from trypsin powder - a mixture of proteases derived from bovine pancreas. Due to itsRead more Have Questions? 联系我们 Quantity: 100 mL Catalog number R001100 Price (TWD)/...
酶解时碳酸氢氨的浓度一般在 10 ～ 50 mM 之间。碳酸氢氨的作用主要是提供一个碱性的水解环境,因为 Typsin 的活力在 pH8 ～ 9 之间最高。最常用的碳酸氢氨浓度有 20 mM , 40 mM 。盐浓度过大时,后续的冻干过程中会有较多的盐析出;盐浓度过小时,则不能有效的提供碱性 pH 的水解环境(尤其是在样品的 ...
Synonyms 500mg;TRYPSIN-EDTA;EC 3.4.21.1;TRYPSIN PORCINE;RecoMbinant Trypsin;trypsin from porcine pancreas;Lys-C;C00298;u-4858;Tryprar CBNumber: CB0673677 Molecular Formula: C35H47N7O10 Molecular Weight: 725.78858 MDL Number: MFCD01323069 MOL File: 9002-07-7.mol MSDS File: SDS Last...
This trypsin solution is modified as follows: With • EDTA • Phenol Red The completeformulationis available. Quality Testing Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots ...
胰蛋白酶(9002-07-7,Trypsin)用作消化液使用,胰蛋白酶的功能主要是使细胞间的蛋白质水解,胰蛋白酶的其他参数见主页|Trypsin (9002-07-7,胰蛋白酶) is used as a digestive solution. The function of trypsin is mainly to hydrolyze the protein between cells. See the h
The Trypsin Profile IGD Kit is composed of 6 reagents: • Destaining Solution – Solution to remove dye bound to the protein of interest. One bottle of powder that reconstitutes to a final volume of 75 ml (Product code D 9940).• Trypsin Reaction Buffer – Buffer provides optimal pH...
2. Add sufficient TPCK Trypsin to the protein solution to have an enzyme: substrate ratio between 1:20 and 1:100 (w/w). 3. Incubate sample for 2–18 hours at 37 degrees C. Note: The exact incubation time and enzyme: substrate ratio will have to be determined for each protein individu...