Sequencing-grade modified porcine trypsin was from Promega (Madison, WI, USA) and sequencing-grade modified bovine chymotrypsin was from Princeton Sepa- ... davidj,agaryk 被引量: 0发表: 0年 Analysis of Subtle Changes in Biological Systems Through use of High Resolution, High Accuracy UHPLC Gen...
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A copy of the spectrogram generated with carbonic anhydrase II and any lot of Trypsin Gold, Mass Spectrometry Grade, may be obtained from Promega Technical Services at 1-800-356-9526. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526...
hourdigestshowsthetwomaindigestionproductsandhasnosignificantnewpeakscomparedwiththe2-hourdigest.PromegaCorporation2800WoodsHollowRoadMadison,WI53711-5399USATelephone***-***-***0TollFree800-356-9526Fax608-277-2516Internet.promegaPRODUCTUSELIMITATIONS, WARRANTY, DISCLAIMER Promega manufactures products for a ...
The amplicons (389 bp for dsPxTryp_SPc1 and 469 bp for dsEGFP) were used as templates for in vitro transcription reactions to produce dsRNAs using the T7 RiboMAX Express RNAi System (Promega, Madison, WI, USA). The synthesized dsRNA was dissolved in 10 mM Tris–HCl (pH 7.0), and 1...
The final PCR products were cloned into pGEM®-T Easy vector (Promega, Madison, WI, USA). An Applied Biosystems DNA sequencer (Model ABI PRISM 377, Shimadzu Corporation, Kyoto, Japan) was used for DNA sequencing. Subsequently, the blast search (NCBI) was performed for sequence alignment ...