Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. This Gibco™ Trypsin/EDTA solution is formulation as 0.025% trypsin and 0.01% EDTA in Phosphate Buffered Saline (PBS). We offer a variety of Gibco™...
Versene solution, an EDTA solution in PBS, dissociates cells gently and non-enzymatically. This chelating agent detaches some cell types (such as epithelial cells) from vessel surfaces for suspension. It can also be used as a wash prior to trypsinization. ...
. The cells were harvested by incubation in trypsin-EDTA solution for 10-15 minutes. Then cells were centrifuged at 300×g for 5 minutes and cell pellets suspended in fresh culture medium prior to seeding into culture flasks or plates
产品名称:Trypsin-EDTA (0.05%), phenol red 适应细胞类型:细胞表面蛋白的完整性很重要的连续细胞系 GIBCO®胰蛋白酶-EDTA为胰蛋白酶粉末,由猪*来源的蛋白酶的照射混合物制成。根据其消化强度,胰蛋白酶被广泛地用于细胞分离,常规的细胞培养传代,和主要组织离解。所需的解离的胰蛋白酶浓度与细胞类型和实验要求...
Recombinant Trypsin Solution is a defined, animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues. This solution is optimized for sensitive cells, such as primary human mesenchymal stem cells (hMSC). ...
SMT-cc1 cells were grown to confluency, removed from the flask by adding 4 mM EDTA and 0.01% DNase solution for 2 min at 23°C, followed by gentle tapping of the tray against the bench. Complete detachment was confirmed by direct visualization. Single-cell suspensions were made by repeated...
aThe mRTEC-iPS cells were dissociated by 0.05% trypsin-EDTA and transferred to low-attachment culture dishes (Corning Inc., USA) in ES medium. mRTECiPS细胞由0.05%胰蛋白乙二胺四乙酸离解并且转移了到低附件培养皿(Corning Inc.,美国)在ES媒介。[translate]...
animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues. The EDTA component accelerates the dissociation action of the trypsin enzyme. This solution is optimized for sensitive cells, such as primary human mesenchymal stem cells (hMSC)...
5A). After fertilization, LsTryp10 was also shown to be present throughout the embryonic cells and in patches associated with yolk granules (Fig. 5C, D). Negative controls were performed without primary antibodies and confirmed specific staining. Relative quantification showed that the LsTryp10 ...
Versene solution Versene solution, an EDTA solution in PBS, dissociates cells gently and non-enzymatically. This chelating agent detaches some cell types (such as epithelial cells) from vessel surfaces for suspension. It can also be used as a wash prior to trypsinization. For further guidance...