(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
A specific anti-TRPA1 primary antibody (anti-TRPA1 polyclonal antibody; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used. The results were normalized to the control group densitometry values and expressed as the relative amount of TRPA1 immunoreactivity. Real-time PCR Spinal cord and ...
For CGRP we used an anti-rabbit antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at a dilution of 1:3200. For SP we used an anti-rabbit antibody (Chemicon, Temecula, CA, USA) at a dilution of 1:5000. After several rinses in a KPBS solution containing 0.04% TX, sections ...
(Invitrogen) for separation by electrophoresis. Proteins were then transferred to a PVDF membrane using the Biorad Trans Blot Turbo Transfer System. Primary antibodies are described in Table1. Secondary antibody goat anti-rabbit IgG-HRP from Santa Cruz were used with rabbit primary antibodies and ...
The mitoTEMPO was obtained from Santa Cruz (Dallas, TX). Laminin was acquired from Life Technologies (Carlsbad, CA). N,N-diisopropylethylamine, 2-bromo-5-methoxytoluene, TBDMS-Cl and PdCl2(dppf)·CH2Cl2 complex (1:1) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Sodium ...
PC-Acro was detected by an antibody against Nε-(3-formyl-3,4-dehydropiperidino)-lysine (FDP-lysine) (NOF corporation, Tokyo, Japan). 4.4. RNA Extraction and qRT-PCR Total RNA was extracted from control and acrolein-treated A549 cells using the NucleoSpin® RNA kit (Takara Bio, Gunma,...
The PVDF membrane was then exposed to the first antibody (TRPA1 (host, goat; Santa Cruz Biotechnology Inc.), 1:1,000 dilution; HIF1α (host, rabbit; Novus Biological), 1:2,000 dilution; or HIF2α (host, rabbit; Novus Biological), 1:2,000 dilution) overnight at 4 °C. The blot...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
Antibody against fibronectin was obtained from Abcam. Antibodies against MIF, Zonula occludens-1, claudin-1 and occludin were obtained from Affinity biosciences (Cincinnati, OH, USA). Antibodies against E-cadherin, MLCK, MLC20 and α-actin were obtained from Santa Cruz (Santa Cruz, CA, USA)....
Toluene diisocyanate (TDI), a major intermediate agent used in the manufacturing industry, causes respiratory symptoms when exposed to the human body. In this study, we aimed to determine the molecular mechanism of TDI toxicity. To investigate the impact