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The assessment of hyperalgesia was measured by the using calibrated von Frey filaments (20PC Aesthe Model, NO. 160615, Muromachi Kikai Co., Ltd. Tokyo, Japan), to the plantar aspect of the hind paw of mice that were kept in suspended wire mesh cages. A response was indicated by lifting ...
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The gene products of both entC and entD are involved in the conversion of isochorismic acid into salicylic acid, and hence correspond to salicylate synthase.doi:10.1111/j.1574-6968.2010.02004.xNivedita NagacharDepartment of Biological Sciences, University of Hull, Hull, UKColin Ratledge...
Monteiro FA, Cardoso I, Sousa MM, Saraiva MJ: In vitro inhibition of transthyretin aggregate-induced cytotoxicity by full and peptide derived forms of the soluble receptor for advanced glycation end products (RAGE). FEBS Lett. 2006, 580: 3451-3456. 10.1016/j.febslet.2006.05.020. Article Pub...
Supernatants were collected, centrifuged 10 min 300 g 4 °C to remove cells and debris, and then total fluorescence was assayed at 463/536 nm with a Tecan Infinite500 spectrophotometric system (Tecan, Group Ltd, Switzerland). Constitutive or evoked MV shedding was quantified from at ...
22. Monteiro FA, Cardoso I, Sousa MM, Saraiva MJ: In vitro inhibition of transthyretin aggregate-induced cytotoxicity by full and peptide derived forms of the soluble receptor for advanced glycation end products (RAGE). FEBS Lett 2006, 580:3451-3456. 23. Subasinghe S, Unabia S, Barrow CJ,...
In order to avoid the negative impact of animal fixation on the experiment, we chose the whole-body exposure to FA at 2.44 ppm for 3 h per day in this study. One exposure chamber (Model WH-2, Yuxin S&T Development Co. Ltd., Wuhan, China) was used to achieve the conversion of liq...
RSV (trans-3, 4′, 5-trihydroxystilbene) is a unique phytoalexin found in plants and fruits such as grapes and grape products. Its strong antioxidant action induced protective action against hypoxia-induced ROS generation and cytotoxicity in several neurons18,19. TRPM2 channel is activated in ...
A fully characterized 293 T-based master cell bank was established at Cell and Gene Therapy at King's (King's College London, London, UK). FEMX-I (human malignant melanoma HLA-A*02:01 positive cell line, originating from a lymph node metastasis in a patient, kindly provided by Professor...