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Sensing and responding to temperature is crucial in biology. The TRPV1 ion channel is a well-studied heat-sensing receptor that is also activated by vanilloid compounds, including capsaicin. Despite significant interest, the molecular underpinnings of th
Assessment of production conditions for efficient use of Escherichia coli in high-yield heterologous recombinant selenoprotein synthesis. Appl Environ Microbiol 2004; 70: 5159–5167. 44. Anestal K, Prast-Nielsen S, Cenas N, Arner ES. Cell death by SecTRAPs: thioredoxin reductase as a prooxidant...
TRPM5 is the first protein identified to show high and invariable enrichment in brush cells of the gastrointestinal tract and the respiratory system. Our data are suggestive of brush cells as a specialized population of epithelial cells potentially involved in chemosensation and of TRPM5 as a ...
Tryptophan-containing 1,5-tetrazole dipeptide analogs: Synthesis of Trpψ[CN4]Nle as a cis amide bond surrogatedoi:10.1016/S0040-4039(00)60771-2The 1,5-disubstituted tetrazole dipeptide analog Z-Trp(NinZ)蠄[CN4]Nle-OBzl was synthesized in good yield and high optical purity using a modified...
TRPV1, with this concentration being recognized as an optimal compromise between channel stimulation and apoptotic triggering [7]. In addition, we chose cyclodextrins as a molecular carrier to remove and load Chol into the sperm membrane compartment because of their high affinity for hydrophobic ...
Subsequent treatment with ammonium acetate in acetic acid under reflux [22] resulted in ring closure to two poorly separable unsaturated lactams, 5a with a Δ5,10- and 5b with a Δ5,6 double bond (yield: 87.4%, ratio 5a:5b: 15:85). Unfortunately, we could not find a suitable oxidant...
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The localizations are highly specific, the difference of a single double bond can restrict the localization of a species to either the mesometrial (M-pole) or antimesometrial (AM-pole) hemisphere of the uterus. For example, PC 36:3 (18:1–18:2) is primarily localized to the M-pole ...
S2). Ultimately, solubilisation hTRPM4-eGFP with 1% LMNG, 2% DDM or 1% digitonin was found to provide the highest yield of oligomeric protein and thus, TRPM4 was purified using mild non-ionic detergents. TRPM4-eGFP purification The TRPM4-eGFP fusion protein was isolated under non-denaturing ...