Cross-linking reaction Cell samples were collected, washed twice with PBS and incubated in PBS pH 8.0 + 1 mM disuccinimidyl suberate (C100015, Sango Biotech, Shanghai, China) for 30 min at room temperature as previously described [25], and subsequent steps were performed as describe...
TRPV1 is an ion channel activated by heat and pungent agents including capsaicin, and has been extensively studied in nociception of sensory neurons. However, the location and function of TRPV1 in the hippocampus is debated. We found that TRPV1 is expres
C a2+ activates transglutaminases [17, 23], which catalyse the cross-linking of glutamine and lysine residues of involucrin and loricrin, a key reaction in the formation of the highly resistant pro- tein envelope. Synthesis of involucrin, loricrin, and a third important corneocyte protein, ...
The capsaicin receptor, transient receptor potential vanilloid type -1 (TRPV1) directs complex roles in signal transduction including the detection of noxious stimuli arising from cellular injury and inflammation. Under pathophysiologic conditions, TRPV1
The reaction was performed at 25 °C in a VersaMax spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), using the same reaction mixture without enzyme as the reference. For each data point, the initial velocity was determined in triplicate over at least five different substrate concentrations...
Primer pairs were designed to target the C-terminal exon, flanking the mCherry or mTagBFP sequence and BLAST-checked against the murine genome to prevent unspecific binding. Mouse GAPDH was used as the reference gene and no-RT-control. The primer sequences are summarized in Table 1. Table ...
Small holes were drilled in the skull and three sterile metal electrodes with mounting screws were implanted epidurally, which comprised a recording electrode over the right hippocampus (anterioposterior, −2.0 mm; mediolateral, 1.6 mm), a reference electrode, and a ground electrode over the ...
Transient receptor potential (TRP) proteins form a superfamily Ca2+-permeable cation channels regulated by a range of chemical and physical stimuli. Structural analysis of a ‘minimal’ TRP vanilloid subtype 1 (TRPV1) elucidated a mechanism of channel ac
In order to obtain lipophilic 17β-estradiol analogs without a C,O-bond at C-3, for which metabolism into the parent hormone should be fully excluded, we converted the phenolic group into the O-triflate to give the useful building block PRU-933. Suzuki–Miyaura cross-coupling of PRU-9 ...
these three siRNA had a cross inhibitory effect: TRPM2-AS—657 removed also 84% of TRPM2-TE molecules, TRPM2-AS—734 removed also 70% of TRPM2-TE transcripts, whereas TRPM2-TE 55 remove 30% of TRPM2-AS RNA. No significant down-regulation of TRPM2-AS and TRPM2-TE transcripts was ...